Filter: AllWBIPIHCIF/ICC

WB analysis of human ileum using 12675-2-AP (same clone as 12675-2-PBS)
human ileum tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.
WB analysis of HeLa using 12675-2-AP (same clone as 12675-2-PBS)
WB result of PPP2R5A antibody (12675-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP2R5A transfected HeLa cells. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.
WB analysis of HeLa using 12675-2-AP (same clone as 12675-2-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.
WB analysis using 12675-2-AP (same clone as 12675-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.
WB analysis of human brain using 12675-2-AP (same clone as 12675-2-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.
WB analysis of human heart using 12675-2-AP (same clone as 12675-2-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.
WB analysis of human kidney using 12675-2-AP (same clone as 12675-2-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.
WB analysis of human lung using 12675-2-AP (same clone as 12675-2-PBS)
human lung tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 12675-2-AP (same clone as 12675-2-PBS)
IP result of anti-PPP2R5A (IP:12675-2-AP, 4ug; Detection:12675-2-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12675-2-AP (same clone as 12675-2-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12675-2-AP (same clone as 12675-2-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.
IF Staining of PC-3 using 12675-2-AP (same clone as 12675-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PPP2R5A antibody (12675-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12675-2-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PPP2R5A Polyclonal antibody, PBS Only

PPP2R5A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12675-2-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

B56A, PP2A B subunit isoform B alpha, PP2A B subunit isoform B56-alpha, PP2A B subunit isoform B'-alpha, PP2A B subunit isoform PR61-alpha

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12675-2-PBS targets PPP2R5A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3360 Product name: Recombinant human PPP2R5A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 188-486 aa of BC022474 Sequence: FVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENINKILPIMFASLYKISKEHWNPTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSILSNTSAE Predict reactive species
Full Name	protein phosphatase 2, regulatory subunit B', alpha isoform
Calculated Molecular Weight	486 aa, 56 kDa
Observed Molecular Weight	56 kDa
GenBank Accession Number	BC022474
Gene Symbol	PPP2R5A
Gene ID (NCBI)	5525
RRID	AB_2252956
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15172
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PPP2R5A(protein phosphatase 2, regulatory subunit B (B56), alpha isoform) is also named as PR61alpha and belongs to the phosphatase 2A regulatory subunit B56 family. B56 alpha subunit gene encodes a cytoplasmic phosphoprotein(PMID:7592815). The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 50 kDa.