PPP4C Polyclonal antibody

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  WB analysis of HEK-293 using 10262-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10262-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10262-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10262-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10262-1-AP

  IP result of anti-PPP4C (IP:10262-1-AP, 4ug; Detection:10262-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IF Staining of HEK-293 using 10262-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PPP4C antibody (10262-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PPP4C Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 10262-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

PPP4, PPH3, PP4C, PP4, PP X

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, mouse kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 3 publications below

Product Information

10262-1-AP targets PPP4C in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0356

  Product name: Recombinant human PPP4C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-307 aa of BC001416

  Sequence: MAEISDLDRQIEQLRRCELIKESEVKALCAKAREILVEESNVQRVDSPVTVCGDIHGQFYDLKELFRVGGDVPETNYLFMGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYDECLRKYGSVTVWRYCTEIFDYLSLSAIIDGKIFCVHGGLSPSIQTLDQIRTIDRKQEVPHDGPMCDLLWSDPEDTTGWGVSPRGAGYLFGSDVVAQFNAANDIDMICRAHQLVMEGYKWHFNETVLTVWSAPNYCYRCGNVAAILELDEHLQKDFIIFEAAPQETRGIPSKKPVADYFL

• Full Name: protein phosphatase 4 (formerly X), catalytic subunit
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC001416
• Gene Symbol: PPP4C
• Gene ID (NCBI): 5531
• RRID: AB_2300020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P60510
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions. The serine/threonine protein phosphatases (PP) are intimately involved in this process. Serine/threonine protein phosphatase X (PPX; also called protein phosphatase 4 (PP4)) are specifically associated with nuclear factor-kappa B (NF-kappa B) p50, c-Rel, and RelA, which are pleiotropic transcription factors that play central roles in the immune and inflammatory responses, as well as apoptosis. Overexpression of PPX stimulated the DNA-binding activity of c-Rel and activated NF-kappa B-mediated transcription. Although the mechanism by which PPX activates Rel/NF-kappa B-mediated transcription is unclear, PPX appeared to act on Rel/NF-kappa B proteins directly through augmentation of c-Rel activity. It is also possible that PPX may dephosphorylate and subsequently activate other c-Rel-associated transcription factors or other kinases regulating I kappaB (e.g. I kappa B kinases or MEKK1). These data suggest that PPX is an activator, but not an inhibitor, of c-Rel/NF-kappa B, which is in contrast to other protein phosphatases.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IP	Genes Dev; Protein phosphatase 4 controls circadian clock dynamics by modulating CLOCK/BMAL1 activity.; Authors - Sabrina Klemz
	WB,IF,IP	Cell Rep; Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression; Authors - Marisa Connell ; KD Validated
mouse		Front Cardiovasc Med; Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.; Authors - Yue Zheng
human	WB	FEBS Lett; Protein phosphatase 4 negatively regulates LPS cascade by inhibiting ubiquitination of TRAF6.; Authors - Chen Lu L
human	WB,IP	Cell Death Differ; AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner; Authors - Weimin Xu
		Mol Cell; Phosphorylation by JNK switches BRD4 functions; Authors - Ballachanda N Devaiah