PPP4C Recombinant monoclonal antibody

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  WB analysis using 86600-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86600-3-RR (PPP4C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 86600-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86600-3-RR (PPP4C antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 86600-3-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 86600-3-RR (PPP4C antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86600-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86600-3-RR against Human PPP4C were performed. The affinity constant is below 1 pM.

PPP4C Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86600-3-RR

Clone No.251478E5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

EC:3.1.3.16, PP X, PP4, PP4C, PPH3

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Tested Applications

• Positive WB detected in: HeLa cells, mouse kidney tissue
• Positive IHC detected in: human colon cancer tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86600-3-RR targets PPP4C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0356

  Product name: Recombinant human PPP4C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-307 aa of BC001416

  Sequence: MAEISDLDRQIEQLRRCELIKESEVKALCAKAREILVEESNVQRVDSPVTVCGDIHGQFYDLKELFRVGGDVPETNYLFMGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYDECLRKYGSVTVWRYCTEIFDYLSLSAIIDGKIFCVHGGLSPSIQTLDQIRTIDRKQEVPHDGPMCDLLWSDPEDTTGWGVSPRGAGYLFGSDVVAQFNAANDIDMICRAHQLVMEGYKWHFNETVLTVWSAPNYCYRCGNVAAILELDEHLQKDFIIFEAAPQETRGIPSKKPVADYFL

• Full Name: protein phosphatase 4 (formerly X), catalytic subunit
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC001416
• Gene Symbol: PPP4C
• Gene ID (NCBI): 5531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P60510
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions. The serine/threonine protein phosphatases (PP) are intimately involved in this process. Serine/threonine protein phosphatase X (PPX; also called protein phosphatase 4 (PP4)) are specifically associated with nuclear factor-kappa B (NF-kappa B) p50, c-Rel, and RelA, which are pleiotropic transcription factors that play central roles in the immune and inflammatory responses, as well as apoptosis. Overexpression of PPX stimulated the DNA-binding activity of c-Rel and activated NF-kappa B-mediated transcription. Although the mechanism by which PPX activates Rel/NF-kappa B-mediated transcription is unclear, PPX appeared to act on Rel/NF-kappa B proteins directly through augmentation of c-Rel activity. It is also possible that PPX may dephosphorylate and subsequently activate other c-Rel-associated transcription factors or other kinases regulating I kappaB (e.g. I kappa B kinases or MEKK1). These data suggest that PPX is an activator, but not an inhibitor, of c-Rel/NF-kappa B, which is in contrast to other protein phosphatases.