PRH1 Polyclonal antibody

PRH1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21460-1-AP

Synonyms

PRPC, Peptide P-C, Parotid proline-rich protein 1/2, Parotid double-band protein, Pa

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Validation Data Gallery

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  WB analysis of HepG2 using 21460-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21460-1-AP (PRH1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IF Staining of HepG2 using 21460-1-AP

  Immunofluorescent analysis of HepG2 cells using 21460-1-AP (PRH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21460-1-AP targets PRH1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRH1 fusion protein Ag14833
• Full Name: proline-rich protein HaeIII subfamily 1
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC064553
• Gene Symbol: PRH1
• Gene ID (NCBI): 5554
• RRID: AB_2878862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRH1, also known as Salivary acidic proline-rich phosphoprotein 1/2, is a 166 amino acid secreted protein whose molecular weight is 17 kDa. It functions as a highly potent inhibitor of calcium phosphate crystal growth. Similar to other PRPs, PRH1 provides a protective and reparative environment for dental enamel, which is important for teeth integrity. This antibody specifically recognises the 17 kDa protein.