AMPK beta 1 Antibody

AMPK beta 1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10308-1-AP

Synonyms

AMPK, AMPK beta 1, AMPK subunit beta 1, AMPKb, HAMPKb, PRKAB1



Tested Applications

Positive WB detected inmouse liver tissue, A431 cells, HEK-293 cells, HeLa cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10308-1-AP targets AMPK beta 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMPK beta 1 fusion protein Ag0301
Full Name protein kinase, AMP-activated, beta 1 non-catalytic subunit
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession number BC001007
Gene symbol PRKAB1
Gene ID (NCBI) 5564
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AMPK beta 1 antibody 10308-1-APDownload protocol
IHC protocol for AMPK beta 1 antibody 10308-1-APDownload protocol
IF protocol for AMPK beta 1 antibody 10308-1-APDownload protocol
IP protocol for AMPK beta 1 antibody 10308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Mol Endocrinol

Metformin reduces glycometabolism of papillary thyroid carcinoma in vitro and in vivo.

Authors - Chen-Tian Shen