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  • KD/KO Validated

PKC Epsilon Polyclonal antibody

PKC Epsilon Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20877-1-AP

Synonyms

PKC epsilon, PKCE, PRKCE, Protein kinase C epsilon type, protein kinase C, epsilon



Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells, rat brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20877-1-AP is PKC Epsilon Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Epsilon fusion protein Ag14976
Full Name protein kinase C, epsilon
Calculated molecular weight 737 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC109033
Gene symbol PRKCE
Gene ID (NCBI) 5581
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IHC protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IF protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IP protocol for PKC Epsilon antibody 20877-1-APDownload protocol
FC protocol for PKC Epsilon antibody 20877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Silencing of Transcription Factor Sp1 Promotes SN1 Transporter Regulation by Ammonia in Mouse Cortical Astrocytes.

Authors - Katarzyna Dąbrowska
mouseWB

J Cell Mol Med

Protein kinase C is involved in the neuroprotective effect of berberine against intrastriatal injection of quinolinic acid-induced biochemical alteration in mice.

Authors - Peng Liu
humanWB

Biochim Biophys Acta Mol Cell Res

Phosphorylation of ELAVL1 (Ser219/Ser316) mediated by PKC is required for erythropoiesis.

Authors - Xianyong Zhou
  • KD Validated
WB

Biochem Biophys Res Commun

Berberine alleviates hepatic lipid accumulation by increasing ABCA1 through the protein kinase C δ pathway.

Authors - Hui Liang
mouse,hamsterWB,IF,IP

Cell Rep

PKCε SUMOylation Is Required for Mediating the Nociceptive Signaling of Inflammatory Pain.

Authors - Xiangteng Zhao
  • KD Validated
ratWB

Neural Regen Res

Chronic stress causes protein kinase C epsilon-aldehyde dehydrogenase 2 signaling pathway perturbation in the rat hippocampus and prefrontal cortex, but not in the myocardium.

Authors - Wen-Yuan Zhang