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PKC epsilon Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 20877-1-AP

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Con: 44 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

PKC epsilon Antibody WB mouse brain tissue 20877-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 20877-1-AP (PKC epsilon antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours

PKC epsilon Antibody IHC human thyroid cancer tissue 20877-1-AP

Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20877-1-AP(PKC epsilon antibody) at dilution of 1:100 (under 10x lens)

PKC epsilon Antibody IHC human thyroid cancer tissue 20877-1-AP

Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20877-1-AP(PKC epsilon antibody) at dilution of 1:100 (under 40x lens)

PKC epsilon Antibody IF mouse brain tissue 20877-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 20877-1-AP (PKC epsilon antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

PKC epsilon Antibody IF mouse brain tissue 20877-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 20877-1-AP (PKC epsilon antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

PKC epsilon Antibody IP SH-SY5Y cells 20877-1-AP

IP Result of anti-PKC epsilon (IP:20877-1-AP, 4ug; Detection:20877-1-AP 1:800) with SH-SY5Y cells lysate 1600ug.

PKC epsilon Antibody FC Jurkat cells 20877-1-AP

1X10^6 Jurkat cells were stained with 0.2ug PKC epsilon antibody (20877-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, SH-SY5Y cells
Positive IP detected in:
SH-SY5Y cells
Positive IHC detected in:
human thyroid cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue
Positive FC detected in:
Jurkat cells
Recommended dilution:
WB : 1:2000-1:6000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PKC epsilon fusion protein Ag14976
Full name:
protein kinase C, epsilon
Calculated molecular weight:
737aa,84 kDa
Observed molecular weight:
84 kDa
GenBank accession number:
BC109033
Gene ID (NCBI):
5581
Gene symbol
PRKCE
Synonyms
PKC epsilon, PKCE, PRKCE, Protein kinase C epsilon type, protein kinase C, epsilon

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