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- KD/KO Validated
PKC Epsilon Polyclonal antibody
PKC Epsilon Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 20877-1-AP
Validation Data Gallery
|Positive WB detected in
|mouse brain tissue, SH-SY5Y cells, rat brain tissue
|Positive IP detected in
|Positive IHC detected in
|mouse brain tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|mouse brain tissue, HeLa cells, U2OS cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
20877-1-AP targets PKC Epsilon in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, hamster
|Host / Isotype
|Rabbit / IgG
|PKC Epsilon fusion protein Ag14976
|protein kinase C, epsilon
|Calculated molecular weight
|737 aa, 84 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
|Product Specific Protocols
|WB protocol for PKC Epsilon antibody 20877-1-AP
|IHC protocol for PKC Epsilon antibody 20877-1-AP
|IF protocol for PKC Epsilon antibody 20877-1-AP
|IP protocol for PKC Epsilon antibody 20877-1-AP
|Click here to view our Standard Protocols
cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning
Thromboxane A2-TP axis promotes adipose tissue macrophages M1 polarization leading to insulin resistance in obesity
J Cell Mol Med
Protein kinase C is involved in the neuroprotective effect of berberine against intrastriatal injection of quinolinic acid-induced biochemical alteration in mice.
Fish Shellfish Immunol
In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.
Mol Biol Cell
MiR-34a regulates blood-tumor barrier function by targeting protein kinase Cɛ.
Oxid Med Cell Longev
p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke.