PKC Epsilon Polyclonal antibody

• Featured Product
• KD/KO Validated

PKC Epsilon Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 20877-1-AP

Synonyms

PKC epsilon, PKCE, PRKCE, Protein kinase C epsilon type, protein kinase C, epsilon

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Validation Data Gallery

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  WB analysis using 20877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20877-1-AP (PKC epsilon antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20877-1-AP (PKC epsilon antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of mouse brain using 20877-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20877-1-AP (PKC epsilon antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of mouse brain using 20877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20877-1-AP (PKC epsilon antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 20877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20877-1-AP (PKC epsilon antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IP experiment of SH-SY5Y using 20877-1-AP

  IP Result of anti-PKC epsilon (IP:20877-1-AP, 4ug; Detection:20877-1-AP 1:800) with SH-SY5Y cells lysate 1600ug.

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  FC experiment of Jurkat using 20877-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug PKC epsilon antibody (20877-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: mouse brain tissue, SH-SY5Y cells, rat brain tissue
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 20877-1-AP is PKC Epsilon Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: hamster, human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKC Epsilon fusion protein Ag14976
• Full Name: protein kinase C, epsilon
• Calculated molecular weight: 737 aa, 84 kDa
• Observed molecular weight: 84 kDa
• GenBank accession number: BC109033
• Gene symbol: PRKCE
• Gene ID (NCBI): 5581
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Silencing of Transcription Factor Sp1 Promotes SN1 Transporter Regulation by Ammonia in Mouse Cortical Astrocytes.; Authors - Katarzyna Dąbrowska
mouse	WB	J Cell Mol Med; Protein kinase C is involved in the neuroprotective effect of berberine against intrastriatal injection of quinolinic acid-induced biochemical alteration in mice.; Authors - Peng Liu
human	WB	Biochim Biophys Acta Mol Cell Res; Phosphorylation of ELAVL1 (Ser219/Ser316) mediated by PKC is required for erythropoiesis.; Authors - Xianyong Zhou ; KD Validated
	WB	Biochem Biophys Res Commun; Berberine alleviates hepatic lipid accumulation by increasing ABCA1 through the protein kinase C δ pathway.; Authors - Hui Liang
mouse，hamster	WB,IF,IP	Cell Rep; PKCε SUMOylation Is Required for Mediating the Nociceptive Signaling of Inflammatory Pain.; Authors - Xiangteng Zhao ; KD Validated
rat	WB	Neural Regen Res; Chronic stress causes protein kinase C epsilon-aldehyde dehydrogenase 2 signaling pathway perturbation in the rat hippocampus and prefrontal cortex, but not in the myocardium.; Authors - Wen-Yuan Zhang