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PKC Epsilon Polyclonal antibody

PKC Epsilon Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 20877-1-AP


PKC epsilon, PKCE, PRKCE, Protein kinase C epsilon type, protein kinase C, epsilon

Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells, rat brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, HeLa cells, U2OS cells
Positive FC detected inJurkat cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20877-1-AP targets PKC Epsilon in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Epsilon fusion protein Ag14976
Full Name protein kinase C, epsilon
Calculated molecular weight 737 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC109033
Gene symbol PRKCE
Gene ID (NCBI) 5581
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IHC protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IF protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IP protocol for PKC Epsilon antibody 20877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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