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PKC Epsilon Polyclonal antibody

PKC Epsilon Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20877-1-AP

Synonyms

PKC epsilon, PKCE, PRKCE, Protein kinase C epsilon type, protein kinase C, epsilon



Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells, rat brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20877-1-AP is PKC Epsilon Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Epsilon fusion protein Ag14976
Full Name protein kinase C, epsilon
Calculated molecular weight 737 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC109033
Gene symbol PRKCE
Gene ID (NCBI) 5581
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IHC protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IF protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IP protocol for PKC Epsilon antibody 20877-1-APDownload protocol
FC protocol for PKC Epsilon antibody 20877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Biol Cell

MiR-34a regulates blood-tumor barrier function by targeting protein kinase Cɛ.

Authors - Wei Zhao
humanWB

Oxid Med Cell Longev

p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke.

Authors - Ming Zhang
mouseWB

Inflamm Bowel Dis

LRRC19 Promotes Permeability of the Gut Epithelial Barrier Through Degrading PKC-ζ and PKCι/λ to Reduce Expression of ZO1, ZO3, and Occludin.

Authors - Xiaomin Su
humanWB,IHC

Biomed Pharmacother

Regorafenib suppresses epidermal growth factor receptor signaling-modulated progression of colorectal cancer.

Authors - Yu-Chang Liu
ratWB

Cell Transplant

Delta Opioid Receptor Activation with Delta Opioid Peptide [d-Ala2, d-Leu5] Enkephalin Contributes to Synaptic Improvement in Rat Hippocampus against Global Ischemia.

Authors - Guangming Zhang
ratWB

Eur J Pharmacol

Protective effects of dioscin against isoproterenol-induced cardiac hypertrophy via adjusting PKCε/ERK-mediated oxidative stress.

Authors - Ruomiao Li