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- KD/KO Validated
PKC Epsilon Polyclonal antibody
PKC Epsilon Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 20877-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, SH-SY5Y cells, rat brain tissue |
Positive IP detected in | SH-SY5Y cells |
Positive IHC detected in | mouse brain tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse brain tissue |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 16 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
The immunogen of 20877-1-AP is PKC Epsilon Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, hamster |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PKC Epsilon fusion protein Ag14976 |
Full Name | protein kinase C, epsilon |
Calculated molecular weight | 737 aa, 84 kDa |
Observed molecular weight | 84 kDa |
GenBank accession number | BC109033 |
Gene symbol | PRKCE |
Gene ID (NCBI) | 5581 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PKC Epsilon antibody 20877-1-AP | Download protocol |
IHC protocol for PKC Epsilon antibody 20877-1-AP | Download protocol |
IF protocol for PKC Epsilon antibody 20877-1-AP | Download protocol |
IP protocol for PKC Epsilon antibody 20877-1-AP | Download protocol |
FC protocol for PKC Epsilon antibody 20877-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Biol Cell MiR-34a regulates blood-tumor barrier function by targeting protein kinase Cɛ. | ||
Oxid Med Cell Longev p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke. | ||
Inflamm Bowel Dis LRRC19 Promotes Permeability of the Gut Epithelial Barrier Through Degrading PKC-ζ and PKCι/λ to Reduce Expression of ZO1, ZO3, and Occludin. | ||
Biomed Pharmacother Regorafenib suppresses epidermal growth factor receptor signaling-modulated progression of colorectal cancer. | ||
Cell Transplant Delta Opioid Receptor Activation with Delta Opioid Peptide [d-Ala2, d-Leu5] Enkephalin Contributes to Synaptic Improvement in Rat Hippocampus against Global Ischemia. | ||
Eur J Pharmacol Protective effects of dioscin against isoproterenol-induced cardiac hypertrophy via adjusting PKCε/ERK-mediated oxidative stress. |