PRKX/PRKY Polyclonal antibody

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  WB analysis using 17985-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17985-1-AP (PRKX/PRKY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PRKX/PRKY Polyclonal Antibody for WB, ELISA

Cat No. 17985-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PRKY, protein kinase, Y linked

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17985-1-AP targets PRKX/PRKY in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12404

  Product name: Recombinant human PRKY protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-277 aa of BC074852

  Sequence: MEAPGPAQAAAAESNSREVTEDAADWAPALCPSPEARSPEAPAYRLQDCDALVTMGTGTFGRVHLVKEKTAKHFFALKVMSIPDVIRRKQEQHVHNEKSVLKEVSHPFLIRLFWTWHEERFLYMLMEYVPGGELFSYLRNRGHFSSTTGLFYSAEIICAIEYLHSKEIVYRDLKPENILLDRDGHIKLTDFGFAKKLVDRTWTLCGTPEYLAPEVIQSKGHGRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILAGKLYFPRHLDFHVKTGRMM

• Full Name: protein kinase, Y-linked
• Calculated Molecular Weight: 277 aa, 32 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC074852
• Gene Symbol: PRKY
• Gene ID (NCBI): 5616
• RRID: AB_3669286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51817
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKY is a member of the cAMP-dependent serine-threonine protein kinase gene family, which appears to encode a protein related to the catalytic subunit of the cAMP-dependent protein kinases, which are key players in the cellular responses to the second messenger cAMP. A chromosomal aberration involving PRKY is a cause of sex reversal disorder. As a homolog with PRKX, the antibody can recognize endogenous levels of PRKY and PRKX.