PrP Polyclonal antibody

PrP Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12555-1-AP

Synonyms

PRNP, PRIP, GSS, CJD, CD230

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Validation Data Gallery

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  WB analysis of mouse brain using 12555-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12555-1-AP (PrP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12555-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12555-1-AP (PrP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12555-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12555-1-AP (PRNP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12555-1-AP

  IP result of anti-PrP (IP:12555-1-AP, 4ug; Detection:12555-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 12555-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12555-1-AP (PrP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 12555-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12555-1-AP (PrP Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 12555-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12555-1-AP (PrP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human gliomas using 12555-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12555-1-AP (PrP Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of SH-SY5Y using 12555-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PrP (12555-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

12555-1-AP targets PrP in WB, IHC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PrP fusion protein Ag3257
• Full Name: prion protein
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC022532
• Gene Symbol: PrP
• Gene ID (NCBI): 5621
• RRID: AB_2237745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prion protein (PRNP) is a ubiquitous membrane glycoprotein whose abnormal self-replicating, misfolded form is widely believed to cause several central nervous system disorders, collectively known as Transmissible Spongiform Encephalopathies (TSE). Prion diseases are TSEs, attributed to conformational conversion of the cellular prion protein (PrPC) into an abnormal conformer that accumulates in the brain. The two isoforms, PrPC and PrPS, have the same primary amino acid sequence and only differ in conformation. While PrPC is composed of 42% α-helix and only 3% β-sheet, PrPSc is composed of 30% α-helix and 43% β-sheet. PrPC converts to its pathogenic isoform when the region corresponding to the residues 108-144 fold into β-sheets. PrPC is very soluble in detergents and easily digested by proteases while the PrPSc is insoluble in detergents and resistant to protease digestion. Prion diseases exist in infectious, sporadic, and genetic forms.

Publications

Species	Application	Title
mouse,human	WB,IP	Neuron; Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.; Authors - Frank F Heisler
human	IHC	Front Mol Biosci; Single-cell transcriptomics uncover the key ferroptosis regulators contribute to cancer progression in head and neck squamous cell carcinoma; Authors - Fei Liu
human	WB	PLoS One; Copper homeostasis-associated gene PRNP regulates ferroptosis and immune infiltration in breast cancer; Authors - Changwei Lin
human	IHC,WB,CoIP	Mol Carcinog; Inhibition of the RBMS1/PRNP axis improves ferroptosis resistance-mediated oxaliplatin chemoresistance in colorectal cancer; Authors - Yini Xu
human	WB	Front Cell Dev Biol; PRNP is a pan-cancer prognostic and immunity-related to EMT in colorectal cancer; Authors - Haifeng Chen
rat	WB	Burns; Exploring the underlying mechanism by transcriptome sequencing in rats with high-voltage electrical burns and the role of iron metabolism; Authors - Jiawen Hao