PROC Polyclonal antibody, PBS Only

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  WB analysis of mouse liver using 25382-1-AP (same clone as 25382-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25382-1-AP (PROC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25382-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 25382-1-AP (same clone as 25382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25382-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 25382-1-AP (same clone as 25382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25382-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 25382-1-AP (same clone as 25382-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PROC antibody (25382-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 25382-1-PBS in a different storage buffer formulation.

PROC Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 25382-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Vitamin K-dependent protein C, Vitamin K dependent protein C, EC:3.4.21.69, Blood coagulation factor XIV, Autoprothrombin IIA

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Product Information

25382-1-PBS targets PROC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11328

  Product name: Recombinant human PROC protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 113-461 aa of BC034377

  Sequence: DGIGSFSCDCRSGWEGRFCQREVSFLNCSLDNGGCTHYCLEEVGWRRCSCAPGYKLGDDLLQCHPAVKFPCGRPWKRMEKKRSHLKRDTEDQEDQVDPRLIDGKMTRRGDSPWQVVLLDSKKKLACGAVLIHPSWVLTAAHCMDESKKLLVRLGEYDLRRWEKWELDLDIKEVFVHPNYSKSTTDNDIALLHLAQPATLSQTIVPICLPDSGLAERELNQAGQETLVTGWGYHSSREKEAKRNRTFVLNFIKIPVVPHNECSEVMSNMVSENMLCAGILGDRQDACEGDSGGPMVASFHGTWFLVGLVSWGEGCGLLHNYGVYTKVSRYLDWIHGHIRDKEAPQKSWAP

• Full Name: protein C (inactivator of coagulation factors Va and VIIIa)
• Calculated Molecular Weight: 461 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC034377
• Gene Symbol: PROC
• Gene ID (NCBI): 5624
• RRID: AB_2880052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04070
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PROC belongs to the peptidase S1 family. PROC is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PMID:25618265). It exerts a protective effect on the endothelial cell barrier function (PMID:25651845).