Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, mouse brain tissue, mouse heart tissue, rat brain tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10664-1-AP targets PRDX3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1062 Product name: Recombinant human PRX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-256 aa of BC007062 Sequence: MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ Predict reactive species |
| Full Name | peroxiredoxin 3 |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 26-28 kDa |
| GenBank Accession Number | BC007062 |
| Gene Symbol | PRDX3 |
| Gene ID (NCBI) | 10935 |
| RRID | AB_2284207 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30048 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PRDX3 antibody 10664-1-AP | Download protocol |
| IHC protocol for PRDX3 antibody 10664-1-AP | Download protocol |
| WB protocol for PRDX3 antibody 10664-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PRDX3 Polyclonal antibody (Cat# 10664-1-AP) has 46 citations. These appear in journals including Cancer Discovery, Nature Metabolism, Nature Communications, Redox Biology, Cell Death & Disease, Cancer Letters, Free Radical Biology and Medicine, Antioxidants, Antioxidant & Redox Signaling, and PLoS One, among others. Research fields span redox biology and oxidative stress, mitochondrial function, ferroptosis, cancer (renal, bladder, breast, prostate, lymphoma), pulmonary fibrosis, neurodegeneration, diabetes, and immunology. Most studies used Western blot, with some IHC and IP applications.
| Species | Application | Title |
|---|---|---|
Cancer Discov Copper Drives Remodeling of Metabolic State and Progression of Clear Cell Renal Cell Carcinoma | ||
Nat Commun Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice | ||
Exp Mol Med YAP1 inhibits the senescence of alveolar epithelial cells by targeting Prdx3 to alleviate pulmonary fibrosis | ||
Redox Biol LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis. | ||
Redox Biol Age-related declines in mitochondrial Prdx6 contribute to dysregulated muscle bioenergetics. |
Reviews
What customers say
The single available review for this product is highly positive. The customer, using the antibody for Western Blot, gave it a perfect 5/5 rating and expressed strong trust in the company, calling it their favorite antibody supplier. Overall, the feedback reflects complete satisfaction with product performance and brand reliability.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Guillermo (Verified Customer) (09-03-2022) | I trust in Proteintech, my favourite antibody company. 5/5 product.
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