PRDX3 Polyclonal antibody

PRDX3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10664-1-AP

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, Peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10664-1-AP targets PRDX3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDX3 fusion protein Ag1062
Full Name peroxiredoxin 3
Calculated molecular weight 27 kDa
Observed molecular weight 26-28 kDa
GenBank accession numberBC007062
Gene symbol PRDX3
Gene ID (NCBI) 10935
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Protocols

Product Specific Protocols
WB protocol for PRDX3 antibody 10664-1-APDownload protocol
IHC protocol for PRDX3 antibody 10664-1-APDownload protocol
IF protocol for PRDX3 antibody 10664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Evid Based Complement Alternat Med

Mechanistic Study of the Phytocompound, 2- β -D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches.

Authors - Jeng-Yuan Shiau
mouseWB

Phytother Res

Casticin prevents DSS induced ulcerative colitis in mice through inhibitions of NF-κB pathway and ROS signaling.

Authors - Jiamei Ma
humanIHC

Clin Exp Immunol

Peroxiredoxins are involved in the pathogenesis of multiple sclerosis and neuromyelitis optica spectrum disorder.

Authors - A Uzawa
mouseIHC

PLoS One

Mitochondrial peroxiredoxin 3 regulates sensory cell survival in the cochlea.

Authors - Chen Fu-Quan FQ
mouseWB

J Endocrinol

NNT is a key regulator of adrenal redox homeostasis and steroidogenesis in male mice.

Authors - Eirini Meimaridou
mouseWB

J Cell Mol Med

PMID: 21251210

Authors - Xi Lei L