PRDX3 Polyclonal antibody

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  WB analysis using 10664-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10664-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10664-1-AP

  WB result of PRDX3 antibody (10664-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells.

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  WB analysis of MCF-7 using 10664-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10664-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 10664-1-AP

  WB result from Qing-Xi Yue, (PMID:20449493); “Control” and “GAD-treated” above the panel represent the control cells and HeLa cells treated with 10 μMGAD for 48 h, respectively. Each blot is the representative result of three independent experiments.

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  IHC staining of human lung cancer using 10664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10664-1-AP (PRDX3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10664-1-AP (PRDX3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10664-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (10664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

PRDX3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10664-1-AP

Publications(34)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

peroxiredoxin 3, MER5, EC:1.11.1.24, AOP-1, AOP1

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, mouse brain tissue, mouse heart tissue, rat brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 30 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

10664-1-AP targets PRDX3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1062

  Product name: Recombinant human PRX3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-256 aa of BC007062

  Sequence: MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ

• Full Name: peroxiredoxin 3
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: BC007062
• Gene Symbol: PRDX3
• Gene ID (NCBI): 10935
• RRID: AB_2284207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30048
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB	Nat Commun; Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice; Authors - Wen Yin
human	WB	Redox Biol; Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization.; Authors - Guoqing Wang ; KD Validated
mouse	WB	Redox Biol; PMID: 32604037; Authors - Anindya Roy Chowdhury
human	WB,IHC	Redox Biol; LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.; Authors - Wen-Su Wei
rat	IF	Oxid Med Cell Longev; Paeoniflorin Upregulates Mitochondrial Thioredoxin of Schwann Cells to Improve Diabetic Peripheral Neuropathy Indicated by 4D Label-Free Quantitative Proteomics.; Authors - Xinwei Yang
human	WB	Mol Cell Proteomics; Proteomics characterization of the cytotoxicity mechanism of ganoderic acid D and computer-automated estimation of the possible drug target network.; Authors - Yue Qing-Xi QX

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guillermo (Verified Customer) (09-03-2022)

I trust in Proteintech, my favourite antibody company. 5/5 product.

• Applications: Western Blot