|Positive WB detected in||HepG2 cells, A431 cells, HeLa cells, MCF-7 cells, Raji cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:3000-1:15000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15219-1-AP targets PSMA7 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PSMA7 fusion protein Ag7389|
|Full Name||proteasome (prosome, macropain) subunit, alpha type, 7|
|Calculated molecular weight||28 kDa|
|Observed molecular weight||28-30 kDa|
|GenBank accession number||BC004427|
|Gene ID (NCBI)||5688|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PSMA7(Proteasome subunit alpha type-7) is also named as HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis.(PMID: 19442227).
|Product Specific Protocols|
|WB protocol for PSMA7 antibody 15219-1-AP||Download protocol|
|IHC protocol for PSMA7 antibody 15219-1-AP||Download protocol|
|IF protocol for PSMA7 antibody 15219-1-AP||Download protocol|
|IP protocol for PSMA7 antibody 15219-1-AP||Download protocol|
|FC protocol for PSMA7 antibody 15219-1-AP||Download protocol|
|Click here to view our Standard Protocols|
ORL J Otorhinolaryngol Relat Spec
Role of the Ubiquitin C-Terminal Hydrolase L1-Modulated Ubiquitin Proteasome System in Auditory Cortex Senescence.
Exp Cell Res
The effect and mechanism of 19S proteasome PSMD11/Rpn6 subunit in D-Galactose induced mimetic aging models.
Cell Physiol Biochem
PSMA7 Directly Interacts with NOD1 and Regulates its Function.
Biochim Biophys Acta Mol Basis Dis
Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.
Mol Cell Proteomics
Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.
Impairment of protein degradation and proteasome function in hereditary neuropathies.