PSMA7 Polyclonal antibody

PSMA7 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15219-1-AP

Synonyms

20S proteasome, C6, HSPC, Proteasome subunit RC6 1, Proteasome subunit XAPC7, PSMA7, RC6 1, XAPC7



Tested Applications

Positive WB detected inHepG2 cells, A431 cells, HeLa cells, MCF-7 cells, Raji cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:15000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 7 publications below

Product Information

15219-1-AP targets PSMA7 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMA7 fusion protein Ag7389
Full Name proteasome (prosome, macropain) subunit, alpha type, 7
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC004427
Gene symbol PSMA7
Gene ID (NCBI) 5688
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMA7(Proteasome subunit alpha type-7) is also named as HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis.(PMID: 19442227).

Protocols

Product Specific Protocols
WB protocol for PSMA7 antibody 15219-1-APDownload protocol
IHC protocol for PSMA7 antibody 15219-1-APDownload protocol
IF protocol for PSMA7 antibody 15219-1-APDownload protocol
IP protocol for PSMA7 antibody 15219-1-APDownload protocol
FC protocol for PSMA7 antibody 15219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

ORL J Otorhinolaryngol Relat Spec

Role of the Ubiquitin C-Terminal Hydrolase L1-Modulated Ubiquitin Proteasome System in Auditory Cortex Senescence.

Authors - Ya Zhang
ratWB

Exp Cell Res

The effect and mechanism of 19S proteasome PSMD11/Rpn6 subunit in D-Galactose induced mimetic aging models.

Authors - Han Wu
humanWB

Cell Physiol Biochem

PSMA7 Directly Interacts with NOD1 and Regulates its Function.

Authors - Yang Liuzhong L
humanWB

Biochim Biophys Acta Mol Basis Dis

Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.

Authors - Diego Sbardella
humanWB

Mol Cell Proteomics

Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.

Authors - Tatjana Goebel
mouseWB

Glia

Impairment of protein degradation and proteasome function in hereditary neuropathies.

Authors - Jordan J S VerPlank