PSMA7 Polyclonal antibody

• Featured Product
• KD/KO Validated

PSMA7 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 15219-1-AP

Synonyms

20S proteasome, C6, HSPC, Proteasome subunit RC6 1, Proteasome subunit XAPC7, PSMA7, RC6 1, XAPC7

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Validation Data Gallery

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  WB analysis of HepG2 using 15219-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15219-1-AP (PSMA7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15219-1-AP

  WB result of PSMA7 antibody (15219-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected HeLa cells.

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  IP experiment of mouse brain using 15219-1-AP

  IP result of anti-PSMA7 (IP:15219-1-AP, 4ug; Detection:15219-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human liver cancer using 15219-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15219-1-AP (PSMA7 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15219-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15219-1-AP (PSMA7 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15219-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15219-1-AP (PSMA7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15219-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug PSMA7 antibody (15219-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:15000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below

Product Information

15219-1-AP targets PSMA7 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMA7 fusion protein Ag7389
• Full Name: proteasome (prosome, macropain) subunit, alpha type, 7
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC004427
• Gene Symbol: PSMA7
• Gene ID (NCBI): 5688
• RRID: AB_2171751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).

Publications

Species	Application	Title
mouse	WB	Brain; Raising cGMP restores proteasome function and myelination in mice with a proteotoxic neuropathy.; Authors - Jordan J S VerPlank
mouse	WB	Glia; Impairment of protein degradation and proteasome function in hereditary neuropathies.; Authors - Jordan J S VerPlank
human	WB	Mol Cell Proteomics; Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.; Authors - Tatjana Goebel
human	WB	Biochim Biophys Acta Mol Basis Dis; Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.; Authors - Diego Sbardella
human	WB	Cell Physiol Biochem; PSMA7 Directly Interacts with NOD1 and Regulates its Function.; Authors - Yang Liuzhong L
rat	WB	Exp Cell Res; The effect and mechanism of 19S proteasome PSMD11/Rpn6 subunit in D-Galactose induced mimetic aging models.; Authors - Han Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Janelle (Verified Customer) (09-12-2022)

Specific to target

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000