|Positive WB detected in||HL-60 cells, mouse kidney tissue, mouse liver tissue, rat spleen tissue, Raji cells, mouse lung tissue, mouse spleen tissue, rat liver tissue|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human spleen tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14544-1-AP targets PSMB9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PSMB9 fusion protein Ag6048|
|Full Name||proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)|
|Calculated molecular weight||23 kDa|
|Observed molecular weight||20-23 kDa|
|GenBank accession number||BC065513|
|Gene ID (NCBI)||5698|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PSMB9, also named as LMP2, PSMB6i and RING12, belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. High expression of PSMB9 associated with the poor prognosis of patients with NPC.
|Product Specific Protocols|
|WB protocol for PSMB9 antibody 14544-1-AP||Download protocol|
|IHC protocol for PSMB9 antibody 14544-1-AP||Download protocol|
|IF protocol for PSMB9 antibody 14544-1-AP||Download protocol|
|IP protocol for PSMB9 antibody 14544-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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