PSMB9 Polyclonal antibody

PSMB9 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14544-1-AP

Synonyms

20S proteasome, beta1i, LMP2, Low molecular mass protein 2, Macropain chain 7, Proteasome chain 7, Proteasome subunit beta 1i, Proteasome subunit beta type 9, PSMB6i, PSMB9, RING12



Tested Applications

Positive WB detected inHL-60 cells, mouse kidney tissue, mouse liver tissue, rat spleen tissue, Raji cells, mouse lung tissue, mouse spleen tissue, rat liver tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14544-1-AP targets PSMB9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMB9 fusion protein Ag6048
Full Name proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
Calculated molecular weight 23 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC065513
Gene symbol PSMB9
Gene ID (NCBI) 5698
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMB9, also named as LMP2, PSMB6i and RING12, belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. High expression of PSMB9 associated with the poor prognosis of patients with NPC.

Protocols

Product Specific Protocols
WB protocol for PSMB9 antibody 14544-1-APDownload protocol
IHC protocol for PSMB9 antibody 14544-1-APDownload protocol
IF protocol for PSMB9 antibody 14544-1-APDownload protocol
IP protocol for PSMB9 antibody 14544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nat Microbiol

PMID: 30988429

Authors - Daisuke Yamane
ratIF

Chin J Cancer

Establishment and characterization of a novel nasopharyngeal carcinoma cell line (SUNE2) from a Cantonese patient.

Authors - Ju-Qin Dong
humanIHC

Asian Pac J Cancer Prev

Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China.

Authors - Zhao Xiaogang X
humanIHC,IF

J Thorac Cardiovasc Surg

Methylation of the promoter of human leukocyte antigen class I in human esophageal squamous cell carcinoma and its histopathological characteristics.

Authors - Qifeng Sun S
mouseWB

Front Pharmacol

Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy.

Authors - Xin Xie
mouse

EMBO J

Proteasomal degradation within endocytic organelles mediates antigen cross-presentation.

Authors - Debrup Sengupta