PSMC4 Polyclonal antibody

PSMC4 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11389-1-AP

Synonyms

MB67 interacting protein, MIP224, PSMC4, S6, Tat binding protein 7, TBP 7, TBP7



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11389-1-AP targets PSMC4 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMC4 fusion protein Ag1943
Full Name proteasome (prosome, macropain) 26S subunit, ATPase, 4
Calculated molecular weight 418 aa, 47 kDa
Observed molecular weight 43-50 kDa
GenBank accession numberBC014488
Gene symbol PSMC4
Gene ID (NCBI) 5704
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMC4(proteasome (prosome, macropain) 26S subunit, ATPase, 4) is also named as TBP7, S6, TBP7, MIP224 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The predicted 458-amino acid TBP7 protein is 57% identical to TBP1 and they can form heterodimers(PMID:8419915).

Protocols

Product Specific Protocols
WB protocol for PSMC4 antibody 11389-1-APDownload protocol
IHC protocol for PSMC4 antibody 11389-1-APDownload protocol
IF protocol for PSMC4 antibody 11389-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biosci Rep

A novel role of proteasomal β1 subunit in tumorigenesis.

Authors - Yuan Fuqiang F
mouseIF

Neuron

Antibody Therapy Targeting RAN Proteins Rescues C9 ALS/FTD Phenotypes in C9orf72 Mouse Model.

Authors - Lien Nguyen