PSMC4 Polyclonal antibody

PSMC4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11389-1-AP


MB67 interacting protein, MIP224, PSMC4, S6, Tat binding protein 7, TBP 7, TBP7

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, SH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11389-1-AP targets PSMC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMC4 fusion protein Ag1943
Full Name proteasome (prosome, macropain) 26S subunit, ATPase, 4
Calculated Molecular Weight 418 aa, 47 kDa
Observed Molecular Weight 43-50 kDa
GenBank Accession NumberBC014488
Gene Symbol PSMC4
Gene ID (NCBI) 5704
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMC4(proteasome (prosome, macropain) 26S subunit, ATPase, 4) is also named as TBP7, S6, TBP7, MIP224 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The predicted 458-amino acid TBP7 protein is 57% identical to TBP1 and they can form heterodimers(PMID:8419915).


Product Specific Protocols
WB protocol for PSMC4 antibody 11389-1-APDownload protocol
IHC protocol for PSMC4 antibody 11389-1-APDownload protocol
IF protocol for PSMC4 antibody 11389-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Antibody Therapy Targeting RAN Proteins Rescues C9 ALS/FTD Phenotypes in C9orf72 Mouse Model.

Authors - Lien Nguyen

Nat Commun

The 20S as a stand-alone proteasome in cells can degrade the ubiquitin tag.

Authors - Indrajit Sahu


Role of Epigallocatechin Gallate in Glucose, Lipid, and Protein Metabolism and L-Theanine in the Metabolism-Regulatory Effects of Epigallocatechin Gallate.

Authors - Ling Lin

Biosci Rep

A novel role of proteasomal β1 subunit in tumorigenesis.

Authors - Yuan Fuqiang F


The Prostaglandin E2 Receptor EP4 Promotes Vascular Neointimal Hyperplasia through Translational Control of Tenascin C via the cAPM/PKA/mTORC1/rpS6 Pathway

Authors - Hu Xu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tatyana (Verified Customer) (03-11-2023)

Was tested in ICC on PFA-fixed human cells. Provides a signal that looks specific (as compared to no primary control). 4% PFA followed by methanol permebealisation; antibody prepared in 5% goat serum in PBST, overnight incubation (no blocking step).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human, SH-SY5Y cells
PSMC4 Antibody Immunofluorescence validation (1:1000 dilution) in Human, SH-SY5Y cells (Cat no:11389-1-AP)