|Positive WB detected in||mouse brain tissue, rat brain tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11389-1-AP targets PSMC4 in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PSMC4 fusion protein Ag1943|
|Full Name||proteasome (prosome, macropain) 26S subunit, ATPase, 4|
|Calculated molecular weight||418 aa, 47 kDa|
|Observed molecular weight||43-50 kDa|
|GenBank accession number||BC014488|
|Gene ID (NCBI)||5704|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PSMC4(proteasome (prosome, macropain) 26S subunit, ATPase, 4) is also named as TBP7, S6, TBP7, MIP224 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The predicted 458-amino acid TBP7 protein is 57% identical to TBP1 and they can form heterodimers(PMID:8419915).
A novel role of proteasomal β1 subunit in tumorigenesis.
Role of Epigallocatechin Gallate in Glucose, Lipid, and Protein Metabolism and L-Theanine in the Metabolism-Regulatory Effects of Epigallocatechin Gallate.
The 20S as a stand-alone proteasome in cells can degrade the ubiquitin tag.
Antibody Therapy Targeting RAN Proteins Rescues C9 ALS/FTD Phenotypes in C9orf72 Mouse Model.