PSMD1 Polyclonal antibody

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  WB analysis using 17709-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17709-1-AP (PSMD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17709-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17709-1-AP (PSMD1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

PSMD1 Polyclonal Antibody for WB, ELISA

Cat No. 17709-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PSMD 1, 26S proteasome non-ATPase regulatory subunit 1

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17709-1-AP targets PSMD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12009

  Product name: Recombinant human PSMD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 606-953 aa of BC094720

  Sequence: AVSDVNDDVRRAAVESLGFILFRTPEQCPSVVSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSIGGIIILKDTSEDIEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD

• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
• Calculated Molecular Weight: 953 aa, 106 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC094720
• Gene Symbol: PSMD1
• Gene ID (NCBI): 5707
• RRID: AB_3085536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99460
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMD1 is a component of the 26S proteasome and contributes to its function regulation. PSMD1 performs an important role in the regulation of numerous cellular processes through the degradation of proteins targeted for breakdown through ubiquitin. PSMD1 has been found to be closely related to a variety of cancers such as lung adenocarcinoma and breast cancer.