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  • KD/KO Validated

PSMD14/POH1 Polyclonal antibody

PSMD14/POH1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12059-1-AP

Synonyms

PAD1, POH1, PSMD14, rpn11



Tested Applications

Positive WB detected inA431 cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12059-1-AP targets PSMD14/POH1 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD14/POH1 fusion protein Ag2694
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC009524
Gene symbol PSMD14
Gene ID (NCBI) 10213
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PSMD14 (POH1, also known as Rpn11/MPR1/S13/CepP1) protein is a metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. PSMD14 is highly expressed in heart and skeletal muscle. In carcinoma cell lines. down-regulation of PSMD14 by siRNA transfection had a considerable impact on cell viability causing cell arrest in the G0-G1 phase, ultimately leading to senescence.

Protocols

Product Specific Protocols
WB protocol for PSMD14/POH1 antibody 12059-1-APDownload protocol
IHC protocol for PSMD14/POH1 antibody 12059-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biochem

Computational and biochemical studies of isothiocyanates as inhibitors of proteasomal cysteine deubiquitinases in human cancer cells.

Authors - Zainab Sabry Othman Ahmed
humanWB

Oncogene

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Authors - Sparks A A
humanWB

Clin Transl Med

MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.

Authors - Mengqing Xiao
  • KD Validated
humanWB

Theranostics

Blockade of deubiquitinating enzyme PSMD14 overcomes chemoresistance in head and neck squamous cell carcinoma by antagonizing E2F1/Akt/SOX2-mediated stemness.

Authors - Chao Jing
  • KD Validated
humanWB, IF

Theranostics

The PSMD14 inhibitor Thiolutin as a novel therapeutic approach for esophageal squamous cell carcinoma through facilitating SNAIL degradation.

Authors - Chao Jing
humanWB, IHC, IF

Nat Commun

POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.

Authors - Boshi Wang
  • KD Validated