PSTPIP1 Polyclonal antibody

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  WB analysis using 11951-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 11951-1-AP (PSTPIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 11951-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11951-1-AP (PSTPIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 11951-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11951-1-AP (PSTPIP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 11951-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11951-1-AP (PSTPIP1 antibody) at dilution of 1:50 (under 10x lens).

PSTPIP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11951-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

H PIP, CD2BP1S, CD2BP1L, CD2BP1, CD2-binding protein 1

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Tested Applications

• Positive WB detected in: THP-1 cells, mouse thymus tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11951-1-AP targets PSTPIP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2551

  Product name: Recombinant human PSTPIP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-349 aa of BC008602

  Sequence: MMPQLQFKDAFWCRDFTAHTGYEVLLQRLLDGRKMCKDMEELLRQRAQAEERYGKELVQIARKAGGQTEINSLRASFDSLKQQMENVGSSHIQLALTLREELRSLEEFRERQKEQRKKYEAVMDRVQKSKLSLYKKAMESKKTYEQKCRDADDAEQAFERISANGHQKQVEKSQNKARQCKDSATEAERVYRQSIAQLEKVRAEWEQEHRTTCEAFQLQEFDRLTILRNALWVHSNQLSMQCVKDDELYEEVRLTLEGCSIDADIDSFIQAKSTGTEPPAPVPYQNYYDREVTPLTSSPGIQPSCGMIKRFSGLLHGSPKTTSLAASAASTETLTPTPERNEGVYTAIA

• Full Name: proline-serine-threonine phosphatase interacting protein 1
• Calculated Molecular Weight: 416 aa, 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC008602
• Gene Symbol: PSTPIP1
• Gene ID (NCBI): 9051
• RRID: AB_10694665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43586
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1), also known as CD2BP1, is a cytoskeleton-associated adaptor protein that links PEST-type phosphatases to their substrates (PMID: 19584923). It is involved in regulation of the actin cytoskeleton. Preferentially expressed in hematopoetic tissues, PSTPIP1 binds to the cytoplasmic tail of CD2, negatively affecting CD2-triggered T cell activation (PMID: 16670297). It has also been shown to interact with ABL1, PTPN18, WAS, CD2AP, PTPN12 and MEFV/pyrin. Mutations in the gene of PSTPIP1 cause PAPA syndrome (Pyogenic sterile Arthritis, Pyoderma gangrenosum, and Acne), an autoinflammatory disease (PMID:11971877; 14595024).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	AMB Express; Quantitative proteomics revealed modulation of macrophages by MetQ gene of Streptococcus suis serotype 2.; Authors - Xiaomeng Pei
human	IHC	Front Genet; Identification of Novel Prognostic Biomarkers Relevant to Immune Infiltration in Lung Adenocarcinoma.; Authors - Zhi Xia