Tested Applications
| Positive WB detected in | THP-1 cells, mouse thymus tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11951-1-AP targets PSTPIP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2551 Product name: Recombinant human PSTPIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC008602 Sequence: MMPQLQFKDAFWCRDFTAHTGYEVLLQRLLDGRKMCKDMEELLRQRAQAEERYGKELVQIARKAGGQTEINSLRASFDSLKQQMENVGSSHIQLALTLREELRSLEEFRERQKEQRKKYEAVMDRVQKSKLSLYKKAMESKKTYEQKCRDADDAEQAFERISANGHQKQVEKSQNKARQCKDSATEAERVYRQSIAQLEKVRAEWEQEHRTTCEAFQLQEFDRLTILRNALWVHSNQLSMQCVKDDELYEEVRLTLEGCSIDADIDSFIQAKSTGTEPPAPVPYQNYYDREVTPLTSSPGIQPSCGMIKRFSGLLHGSPKTTSLAASAASTETLTPTPERNEGVYTAIA Predict reactive species |
| Full Name | proline-serine-threonine phosphatase interacting protein 1 |
| Calculated Molecular Weight | 416 aa, 48 kDa |
| Observed Molecular Weight | 45-48 kDa |
| GenBank Accession Number | BC008602 |
| Gene Symbol | PSTPIP1 |
| Gene ID (NCBI) | 9051 |
| RRID | AB_10694665 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1), also known as CD2BP1, is a cytoskeleton-associated adaptor protein that links PEST-type phosphatases to their substrates (PMID: 19584923). It is involved in regulation of the actin cytoskeleton. Preferentially expressed in hematopoetic tissues, PSTPIP1 binds to the cytoplasmic tail of CD2, negatively affecting CD2-triggered T cell activation (PMID: 16670297). It has also been shown to interact with ABL1, PTPN18, WAS, CD2AP, PTPN12 and MEFV/pyrin. Mutations in the gene of PSTPIP1 cause PAPA syndrome (Pyogenic sterile Arthritis, Pyoderma gangrenosum, and Acne), an autoinflammatory disease (PMID:11971877; 14595024).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PSTPIP1 antibody 11951-1-AP | Download protocol |
| WB protocol for PSTPIP1 antibody 11951-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PSTPIP1 Polyclonal antibody (Cat# 11951-1-AP) has 3 total citations published in AMB Express, Frontiers in Genetics, and Biochemical Genetics. The cited research spans bacterial infection and macrophage modulation, prognostic biomarker identification in lung adenocarcinoma with immune infiltration, and PSTPIP1 expression analysis in renal cell carcinoma for prognostic value. Applications used include Western blot and immunohistochemistry in both mouse and human samples.
| Species | Application | Title |
|---|---|---|
AMB Express Quantitative proteomics revealed modulation of macrophages by MetQ gene of Streptococcus suis serotype 2. | ||
Front Genet Identification of Novel Prognostic Biomarkers Relevant to Immune Infiltration in Lung Adenocarcinoma. | ||
