PTGER3 Polyclonal antibody

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  WB analysis of mouse brain using 24761-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24761-1-AP (PTGER3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 24761-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 24761-1-AP (PTGER3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 24761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24761-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 24761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24761-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 40x lens).

PTGER3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24761-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

EP3, EP3 I, EP3 II, EP3 III, EP3 IV

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

24761-1-AP targets PTGER3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21542

  Product name: Recombinant human PTGER3 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-100 aa of BC024229

  Sequence: MKETRGYGGDAPFCTRLNHSYTGMWAPERSAEARGNLTRPPGSGEDCGSVSVAFPITMLLTGFVGNALAMLLVSRSYRRRESKRKKSFLLCIGWLALTDL

• Full Name: prostaglandin E receptor 3 (subtype EP3)
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40-48 kDa
• GenBank Accession Number: BC024229
• Gene Symbol: PTGER3
• Gene ID (NCBI): 5733
• RRID: AB_2879710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43115
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGER3 (Prostaglandin E Receptor 3), also known as EP3, is a G protein-coupled receptor (GPCR). Activates pathways involved in proliferation, migration, and apoptosis, often via cross-talk with PI3K-AKT and MAPK cascades (PMID: 38566528). High PTGER3 levels drive tumorigenesis, angiogenesis, and cisplatin resistance via the Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis (PMID: 30655206).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	Dev Cell; Chromatin remodeling of prostaglandin signaling in smooth muscle enables mouse embryo passage through the female reproductive tract; Authors - Qiliang Xin
mouse,human	WB	Cell Metab; m6A mRNA methylation in brown fat regulates systemic insulin sensitivity via an inter-organ prostaglandin signaling axis independent of UCP1; Authors - Ling Xiao