Prothymosin Alpha Polyclonal antibody

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  IHC staining of human thyroid cancer using 10480-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10480-1-AP (Prothymosin Alpha antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 10480-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 10480-1-AP (Prothymosin Alpha antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 10480-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10480-1-AP (Prothymosin alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 10480-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10480-1-AP (Prothymosin alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Prothymosin Alpha Polyclonal Antibody for IHC, ELISA

Cat No. 10480-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, ELISA

Synonyms

Prothymosin alpha, N-terminally processed, prothymosin, alpha, PTMA, Thymosin alpha-1

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Tested Applications

• Positive IHC detected in: human thyroid cancer tissue, human lymphoma tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10480-1-AP targets Prothymosin Alpha in IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Prothymosin Alpha fusion protein Ag0732
• Full Name: prothymosin, alpha
• Calculated Molecular Weight: 12 kDa
• GenBank Accession Number: BC003510
• Gene Symbol: Prothymosin Alpha/PTMA
• Gene ID (NCBI): 5757
• RRID: AB_2174388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P06454
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prothymosin alpha (PTMA) is a nuclear protein possessing a number of cellular functions including cell survival. PTMA is identified to be localized in the nuclei of neurons, while it is found in both nuclei and cytoplasm in the astrocytes and microglia of adult brai. PTMA inhibits the neuronal necrosis induced by serum-free starvation or ischemia-reperfusion stress, which causes a rapid internalization of GLUT1/4, leading a decrease in glucose uptake and cellular ATP levels. Extensive research on PTMA showed that it is of clinical significance and potential medical use as they may serve as a molecular marker for cancer prognosis and/or as therapeutic agents for treating immunodeficiencies, autoimmune diseases and malignancies.