|Positive WB detected in||Sp2/0 cells, BxPC-3 cells, A431 cells, HeLa cells, Jurkat cells, THP-1 cells, mouse liver tissue, human testis tissue, HL-60 cells, human placenta tissue, C6 cells, NIH/3T3 cells, mouse placenta tissue, rat liver tissue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||BxPC-3 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11334-1-AP targets PTP1B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PTP1B fusion protein Ag1878|
|Full Name||protein tyrosine phosphatase, non-receptor type 1|
|Calculated molecular weight||435 aa, 50 kDa|
|Observed molecular weight||45-55 kDa|
|GenBank accession number||BC015660|
|Gene ID (NCBI)||5770|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).
|Product Specific Protocols|
|WB protocol for PTP1B antibody 11334-1-AP||Download protocol|
|IHC protocol for PTP1B antibody 11334-1-AP||Download protocol|
|IF protocol for PTP1B antibody 11334-1-AP||Download protocol|
|IP protocol for PTP1B antibody 11334-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Highly Selective Protein Tyrosine Phosphatase Inhibitor, 2,2',3,3'-Tetrabromo-4,4',5,5'-tetrahydroxydiphenylmethane, Ameliorates Type 2 Diabetes Mellitus in BKS db Mice.
Biochem Biophys Res Commun
HDAC6 interacts with PTPN1 to enhance melanoma cells progression.
Aging (Albany NY)
Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.
Therapeutic Role of Protein Tyrosine Phosphatase 1B in Parkinson's Disease via Antineuroinflammation and Neuroprotection In Vitro and In Vivo.
Cell Death Dis
The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy.