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  • KD/KO Validated

PTP1B Polyclonal antibody

PTP1B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11334-1-AP

Synonyms

PTP 1B, PTP1B, PTPN1



Tested Applications

Positive WB detected inSp2/0 cells, BxPC-3 cells, A431 cells, HeLa cells, Jurkat cells, THP-1 cells, mouse liver tissue, human testis tissue, HL-60 cells, human placenta tissue, C6 cells, NIH/3T3 cells, mouse placenta tissue, rat liver tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11334-1-AP targets PTP1B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTP1B fusion protein Ag1878
Full Name protein tyrosine phosphatase, non-receptor type 1
Calculated molecular weight 435 aa, 50 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC015660
Gene symbol PTPN1
Gene ID (NCBI) 5770
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).

Protocols

Product Specific Protocols
WB protocol for PTP1B antibody 11334-1-APDownload protocol
IHC protocol for PTP1B antibody 11334-1-APDownload protocol
IF protocol for PTP1B antibody 11334-1-APDownload protocol
IP protocol for PTP1B antibody 11334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Cell Physiol

Circular RNA circTET3 mediates migration of rat vascular smooth muscle cells by targeting miR-351-5p.

Authors - Qing-Ping Yao
mouseWB,IHC

FASEB J

PMID: 32100381

Authors - Ping Yang
humanWB

Front Cell Dev Biol

Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma.

Authors - Juan Du
  • KD Validated
mouseWB

iScience

Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis

Authors - Shohei Takaoka
human,mouseWB

Front Pharmacol

Ferulic Acid Ameliorates Hepatic Inflammation and Fibrotic Liver Injury by Inhibiting PTP1B Activity and Subsequent Promoting AMPK Phosphorylation.

Authors - Jianzhi Wu
humanWB

Obesity (Silver Spring)

Suppression of RIP1 activity via S415 dephosphorylation ameliorates obesity-related hepatic insulin resistance.

Authors - Yanping Wang