PTPN12 Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP00788-4

  Sandwich ELISA standard curve of MP00788-4, Human PTPN12 Recombinant Matched Antibody Pair - PBS only. 83851-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35382. 83851-5-PBS was HRP conjugated as the detection antibody. Range: 93.75-6000 pg/mL

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  Sample test of MP00788-4

  The mean PTPN12 concentration was determined to be 18.88 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 21.55 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load and 29.01 ng/mL in U2OS cell extract based on a 1.3 mg/mL extract load.

PTPN12 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83851-5-PBS

Clone No.240983E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

PTP PEST, PTPG1

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Product Information

83851-5-PBS targets PTPN12 as part of a matched antibody pair:

MP00788-4: 83851-1-PBS capture and 83851-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag35382

  Product name: Recombinant human PTPN12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 603-780 aa of NM_002835.3

  Sequence: SDDSDSDERNSDGAVTQNKTNISTASATVSAATSTESISTRKVLPMSIARHNIAGTTHSGAEKDVDVSEDSPPPLPERTPESFVLASEHNTPVRSEWSELQSQERSEQKKSEGLITSENEKCDHPAGGIHYEMCIECPPTFSDKREQISENPTEATDIGFGNRCGKPKGPRDPPSEWT

• Full Name: protein tyrosine phosphatase, non-receptor type 12
• Calculated Molecular Weight: 88kDa
• GenBank Accession Number: NM_002835.3
• Gene Symbol: PTPN12
• Gene ID (NCBI): 5782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q05209
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.