PTPN22 Polyclonal antibody

PTPN22 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11783-1-AP

Synonyms

Lymphoid phosphatase, LYP, LYP1, LYP2, PEP, PTPN22, PTPN8



Tested Applications

Positive WB detected inDaudi cells, Raji cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11783-1-AP targets PTPN22 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPN22 fusion protein Ag2397
Full Name protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
Calculated molecular weight 82 kDa, 92 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC017785
Gene symbol PTPN22
Gene ID (NCBI) 26191
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.

Protocols

Product Specific Protocols
WB protocol for PTPN22 antibody 11783-1-APDownload protocol
IHC protocol for PTPN22 antibody 11783-1-APDownload protocol
IP protocol for PTPN22 antibody 11783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Pflugers Arch

Anandamide produced by Ca(2+)-insensitive enzymes induces excitation in primary sensory neurons.

Authors - Varga Angelika A
humanWB

Sci Rep

TRAF3 enhances TCR signaling by regulating the inhibitors Csk and PTPN22.

Authors - Alicia M Wallis
mouseWB

J Exp Med

NLR Nod1 signaling promotes survival of BCR-engaged mature B cells through up-regulated Nod1 as a positive outcome.

Authors - Kyoko Hayakawa