IA-2/PTPRN Polyclonal antibody

• 

  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10584-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10584-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10584-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10584-1-AP

  IP result of anti-IA-2/PTPRN (IP:10584-1-AP, 3ug; Detection:10584-1-AP 1:600) with mouse brain tissue lysate 7000ug.

• 

  IHC staining of rat pancreas using 10584-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 10584-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10584-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 10584-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse pancreas using 10584-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using IA-2/PTPRN antibody (10584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 10584-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human IA-2/PTPRN (10584-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IA-2/PTPRN Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Cat No. 10584-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Synonyms

IA-2, PTPRN, ICA 512, ICA512-cleaved cytosolic fragment, ICA512-N-terminal fragment

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, mouse kidney tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: rat pancreas tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse pancreas tissue
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

10584-1-AP targets IA-2/PTPRN in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0923

  Product name: Recombinant human PTPRN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 510-811 aa of BC007713

  Sequence: WCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ

• Full Name: protein tyrosine phosphatase, receptor type, N
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 60-65 kDa, 106-115 kDa
• GenBank Accession Number: BC007713
• Gene Symbol: IA-2/PTPRN
• Gene ID (NCBI): 5798
• RRID: AB_2237983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16849
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell).(PMID: 10027571). IA-2/PTPRN was found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Front Cell Dev Biol; Overexpression of PTPRN Promotes Metastasis of Lung Adenocarcinoma and Suppresses NK Cell Cytotoxicity.; Authors - Xinyue Song
human	WB	J Biol Chem; Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.; Authors - Chengfeng Merriman
		PLoS One; Sensitive detection of multiple islet autoantibodies in type 1 diabetes using small sample volumes by agglutination-PCR.; Authors - Felipe de Jesus Cortez
human	IHC	Cell Physiol Biochem; Prognostic Value of Phosphotyrosine Phosphatases in Hepatocellular Carcinoma.; Authors - Guangyan Zhangyuan
human	IHC	Transl Lung Cancer Res; Construction and validation of a prognostic model based on oxidative stress-related genes in non-small cell lung cancer (NSCLC): predicting patient outcomes and therapy responses; Authors - Dongfeng Sun