PUF-A,KIAA0020 Monoclonal antibody

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  WB analysis using 67941-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HSC-T6 using 67941-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 67941-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67941-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67941-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PUF-A,KIAA0020 Monoclonal Antibody for WB, ELISA

Cat No. 67941-1-Ig

Clone No.1G4B7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

HLA HA8, KIAA0020, PEN, PUF6, PUF-A, PUM3, XTP5

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, k-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67941-1-Ig targets PUF-A,KIAA0020 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31324

  Product name: Recombinant human KIAA0020 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 283-648 aa of BC016137

  Sequence: YKSADHPTLDKVLELQPEKLELIMDEMKQILTPMAQKEAVIKHSLVHKVFLDFFTYAPPKLRSEMIEAIREAVVYLAHTHDGARVAMHCLWHGTPKDRKVIVKTMKTYVEKVANGQYSHLVLLAAFDCIDDTKLVKQIIISEIISSLPSIVNDKYGRKVLLYLLSPRDPAHTVREIIEVLQKGDGNAHSKKDTEVRRRELLESISPALLSYLQEHAQEVVLDKSACVLVSDILGSATGDVQPTMNAIASLAATGLHPGGKDGELHIAEHPAGHLVLKWLIEQDKKMKENGREGCFAKTLVEHVGMKNLKSWASVNRGAIILSSLLQSCDLEVANKVKAALKSLIPTLEKTKSTSKGIEILLEKLST

• Full Name: KIAA0020
• Calculated Molecular Weight: 648 aa, 74 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC016137
• Gene Symbol: PUF-A/KIAA0020
• Gene ID (NCBI): 9933
• RRID: AB_2918693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15397
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.