PVRIG Polyclonal antibody

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• PVRIG Polyclonal antibody (21448-1-AP)

PVRIG Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21448-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

Transmembrane protein PVRIG, Poliovirus receptor-related immunoglobulin domain-containing protein, CD112R, CD112 receptor, C7orf15

• 

  WB analysis using 21448-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21448-1-AP (PVRIG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 21448-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 21448-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 21448-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MOLT-4 cells, NK-92 cells, Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21448-1-AP targets PVRIG in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13815

  Product name: Recombinant human PVRIG protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 193-326 aa of BC001129

  Sequence: LRRHKHRPAPRLQPSRTSPQAPRARAWAPSQASQAALHVPYATINTSCRPATLDTAHPHGGPSWWASLPTHAAHRPQGPAAWASTPIPARGSFVSVENGLYAQAGERPPHTGPGLTLFPDPRGPRAMEGPLGVR

• Full Name: poliovirus receptor related immunoglobulin domain containing
• Calculated Molecular Weight: 326 aa, 34 kDa
• Observed Molecular Weight: ~34 kDa
• GenBank Accession Number: BC001129
• Gene Symbol: PVRIG
• Gene ID (NCBI): 79037
• RRID: AB_2878859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6DKI7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PVRIG, also named as CD112R and C7orf15, is an immune suppressive molecule expressed on T and NK cells. It's a cell surface receptor for NECTIN2. PVRIG is a target for the treatment of cancer.

Publications

What published studies show

The PVRIG Polyclonal antibody (Cat# 21448-1-AP) has 1 total citation. It appears in Cell Death & Disease (IF 12.2), a 2026 study by Lihua Chen et al. The research investigates CD112's (PVRIG) spatiotemporal expression and its dual role in T-cell exhaustion and ferroptosis crosstalk in cervical cancer, using immunofluorescence (IF) on human samples. The associated fields are cancer immunology, T-cell biology, and ferroptosis.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell Death Dis; Spatiotemporal mapping of CD112 unveils its dichotomous role in T-cell exhaustion and ferroptosis crosstalk in cervical cancer.; Authors - Lihua Chen