PVRIG Polyclonal antibody

PVRIG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21448-1-AP

Synonyms

Transmembrane protein PVRIG, Poliovirus receptor-related immunoglobulin domain-containing protein, CD112R, CD112 receptor, C7orf15

---

Validation Data Gallery

• 

  WB analysis using 21448-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21448-1-AP (PVRIG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 21448-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21448-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21448-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21448-1-AP (PVRIG antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: MOLT-4 cells, NK-92 cells, Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21448-1-AP targets PVRIG in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PVRIG fusion protein Ag13815
• Full Name: poliovirus receptor related immunoglobulin domain containing
• Calculated Molecular Weight: 326 aa, 34 kDa
• Observed Molecular Weight: ~34 kDa
• GenBank Accession Number: BC001129
• Gene Symbol: PVRIG
• Gene ID (NCBI): 79037
• RRID: AB_2878859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PVRIG, also named as CD112R and C7orf15, is an immune suppressive molecule expressed on T and NK cells. It's a cell surface receptor for NECTIN2. PVRIG is a target for the treatment of cancer.