Paxillin Polyclonal antibody

Paxillin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 10029-1-Ig

Synonyms

paxillin, PXN



Tested Applications

Positive WB detected inmouse brain tissue, COLO 320 cells, Jurkat cells, MCF-7 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells, NIH/3T3 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10029-1-Ig targets Paxillin in WB, IP, IHC, IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name paxillin
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberNM_002859
Gene symbol PXN
Gene ID (NCBI) 5829
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PXN (paxillin) is a 68 kDa scaffold protein that interacts with multiple structural and signaling proteins and regulates cell adhesion, migration, proliferation, and apoptosis. PXN is thought to play an important role in tumor migration, invasion, and metastasis (21045234). PXN has been identified as a direct substrate of protein tyrosine phosphatase receptor-type T (PTPRT), a potent tumor suppressor gene. Increased phospho-PXN at tyrosine residue 88 (Y88) has been found as a common feature of human colon cancers (20133777).

Protocols

Product Specific Protocols
WB protocol for Paxillin antibody 10029-1-IgDownload protocol
IHC protocol for Paxillin antibody 10029-1-IgDownload protocol
IF protocol for Paxillin antibody 10029-1-IgDownload protocol
IP protocol for Paxillin antibody 10029-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Acta Pharmacol Sin

Reduced intracellular chloride concentration impairs angiogenesis by inhibiting oxidative stress-mediated VEGFR2 activation.

Authors - Kai Li
humanWB

J Mater Sci Mater Med

Surface changes of nanotopography by carbon ion implantation to enhance the biocompatibility of silicone rubber: an in vitro study of the optimum ion fluence and adsorbed protein.

Authors - Xianhui Li
humanWB

Cancer Med

SphK1-driven autophagy potentiates focal adhesion paxillin-mediated metastasis in colorectal cancer.

Authors - Jiang-Ni Wu
mouseIF

Mol Neurobiol

Essential Role of Sptan1 in Cochlear Hair Cell Morphology and Function Via Focal Adhesion Signaling.

Authors - Qingxiu Yao
humanIF

Sci Signal

Mitotic cell responses to substrate topological cues are independent of the molecular nature of adhesion.

Authors - Ouranio Anastasiou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (03-11-2019)

For WB, besides the expected band, it also recognised some other non-specific bands; for IF, it could label the cell cortex localisation, partially co-localised with Actin.

  • Applications: Western Blot,Immunofluorescence,
  • Cell Tissue Type: MEF