PYCR2-specific Polyclonal antibody

PYCR2-specific Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF

Conjugate

Unconjugated

Publications

1

Cat no : 55060-1-AP

Synonyms

P5C reductase 2, P5CR 2, P5CR2, PYCR2

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Validation Data Gallery

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  WB analysis of HeLa using 55060-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55060-1-AP (PYCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 55060-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55060-1-AP (PYCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 55060-1-AP

  Immunofluorescent analysis of HeLa cells using 55060-1-AP (PYCR2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55060-1-AP targets PYCR2-specific in WB, IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: pyrroline-5-carboxylate reductase family, member 2
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 27-31 kDa, 20 kDa
• GenBank Accession Number: NM_013328
• Gene Symbol: PYCR2
• Gene ID (NCBI): 29920
• RRID: AB_10888635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody is specific to PYCR2 and has no cross reaction to PYCR1.

Publications

Species	Application	Title
human	WB	Sci Rep; Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.; Authors - Bobby Jones