PYCR2-specific Polyclonal antibody

PYCR2-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55060-1-AP

Synonyms

PYCR2, PYCR 2, P5CR2, P5CR 2, HLD10



Tested Applications

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55060-1-AP targets PYCR2-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name pyrroline-5-carboxylate reductase family, member 2
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 27-31 kDa, 20 kDa
GenBank Accession NumberNM_013328
Gene Symbol PYCR2
Gene ID (NCBI) 29920
RRIDAB_10888635
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody is specific to PYCR2 and has no cross reaction to PYCR1.

Protocols

Product Specific Protocols
WB protocol for PYCR2-specific antibody 55060-1-APDownload protocol
IHC protocol for PYCR2-specific antibody 55060-1-APDownload protocol
IF protocol for PYCR2-specific antibody 55060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Rep

Proline restores mitochondrial function and reverses aging hallmarks in senescent cells

Authors - Debanik Choudhury
humanWB

Sci Rep

Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.

Authors - Bobby Jones