PYCR2-specific Polyclonal antibody

PYCR2-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55060-1-AP

Synonyms

PYCR2, PYCR 2, P5CR2, P5CR 2, HLD10

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Validation Data Gallery

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  WB analysis of HeLa using 55060-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55060-1-AP (PYCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 55060-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55060-1-AP (PYCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 55060-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55060-1-AP (PYCR2-specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 55060-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PYCR2-specific antibody (55060-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

55060-1-AP targets PYCR2-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: pyrroline-5-carboxylate reductase family, member 2
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 27-31 kDa, 20 kDa
• GenBank Accession Number: NM_013328
• Gene Symbol: PYCR2
• Gene ID (NCBI): 29920
• RRID: AB_10888635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody is specific to PYCR2 and has no cross reaction to PYCR1.

Publications

Species	Application	Title
human	WB,IF	Cell Rep; Proline restores mitochondrial function and reverses aging hallmarks in senescent cells; Authors - Debanik Choudhury
human	WB	Sci Rep; Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.; Authors - Bobby Jones
human	WB	Int J Mol Sci; Deciphering the Effects of the PYCR Family on Cell Function, Prognostic Value, Immune Infiltration in ccRCC and Pan-Cancer; Authors - Hongquan Chen ; KD Validated