Phospho-JUN (Ser73) Polyclonal antibody

Phospho-JUN (Ser73) Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 28891-1-AP

Synonyms

Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, P39, Phospho-JUN (Ser73), Proto oncogene c Jun, Transcription factor AP 1

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Validation Data Gallery

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  WB analysis using 28891-1-AP

  Non-treated NIH/3T3 and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28891-1-AP (Phospho-JUN (Ser73) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of NIH/3T3 using 28891-1-AP

  Immunofluorescent analysis of (4% PFA) fixed UV treated and non-treated NIH/3T3 cells using Phospho-JUN (Ser73) antibody (28891-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: UV treated NIH/3T3 cells
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below

Product Information

28891-1-AP targets Phospho-JUN (Ser73) in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: jun oncogene
• Calculated Molecular Weight: 331 aa, 36 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC068522
• Gene Symbol: JUN
• Gene ID (NCBI): 3725
• RRID: AB_2881228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis (PMID: 22180088). JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription (PMID: 9732876). In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression (PMID:8464713). More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins (PMID: 15469925). This antibody is raised against synthetic phosphopeptide corresponding to residues surrounding Ser73 of human JUN, which can detect the bands around 42-45 kDa.

Publications

Species	Application	Title
mouse	WB	Biochem Pharmacol; Activation of CXCL13/CXCR5 axis aggravates experimental autoimmune cystitis and interstitial cystitis/bladder pain syndrome.; Authors - Jiang Zhao
human	WB	Int J Biol Sci; RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway.; Authors - Deyu Zhang
mouse	WB	Front Pharmacol; Azithromycin Attenuates Bleomycin-Induced Pulmonary Fibrosis Partly by Inhibiting the Expression of LOX and LOXL-2.; Authors - Xiang Tong
pig	WB	J Appl Microbiol; Enterococcus faecium HDRsEf1 inhibits LPS-induced downregulation of ZO-1 expression via TLR2/4-mediated JNK/AP-1 signalling pathways.; Authors - Lin Liu
human	WB	Cell Death Dis; Usp8 promotes tumor cell migration through activating the JNK pathway.; Authors - Yunhe Zhao
human	WB	Cell Mol Biol Lett; Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway; Authors - Zhaoyi Liang