Phospho-JUN (Ser73) Polyclonal antibody

Phospho-JUN (Ser73) Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 28891-1-AP

Synonyms

Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, JUN S73, JUN Ser73, P39, Phospho-JUN (Ser73), Proto oncogene c Jun, S73), Ser73, Transcription factor AP 1



Tested Applications

Positive WB detected inUV treated NIH/3T3 cells
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

28891-1-AP targets Phospho-JUN (Ser73) in WB, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name jun oncogene
Calculated molecular weight 331 aa, 36 kDa
Observed molecular weight38-45 kDa
GenBank accession numberBC068522
Gene symbol JUN
Gene ID (NCBI) 3725
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis[PMID: 22180088]. JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription[PMID: 9732876]. In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression[PMID:8464713]. More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins[PMID: 15469925]. This antibody is a rabbit polyclonal antibody raised against a region of human JUN. Both phosphorylated (p-c-Jun) and unphosphorylated forms of c-Jun, with sizes of 42-45 kDa and 36-39 kDa, respectively are obtain in some experiments. (PMID: 17210646)

Protocols

Product Specific Protocols
WB protocol for Phospho-JUN (Ser73) antibody 28891-1-APDownload protocol
IF protocol for Phospho-JUN (Ser73) antibody 28891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols