Phosphatase Inhibitor Cocktail (100X)

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  NIH3T3 cells were activated with 400 nM PMA for 15 min. Cells were then lysed using RIPA Buffer (PR20135) with (lanes 1 and 2) and without (lanes 3 and 4) phosphatase inhibitor (PR20015). Cell lysates were then incubated at 37°C for 4 hours (lanes 1 and 3) or 21 hours (lanes 2 and 4). Following this incubation lysates were subjected to SDS-PAGE (5 ug protein/well) followed by western blot with anti-phospho-ERK1/2 (Thr202/Tyr204; 28733-1-AP).

Cat No. PR20015

Publications

25

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Product Information

Proteintech's Phosphatase Inhibitor Cocktail is a highly potent, broad-spectrum cocktail containing multiple phosphatase inhibitors. The main components include sodium fluoride, sodium orthovanadate, sodium pyrophosphate, beta-glycerophosphate, imidazole, sodium molybdate, sodium tartrate dibasic dihydrate, and other inhibitors, mainly inhibiting serine/threonine phosphatases, acid phosphatases, alkaline phosphatases, tyrosine phosphotases, etc. The cocktail can effectively prevent protein dephosphorylation during the extraction or lysis of primary cells, mammalian cultured cells, animal tissues, plant tissues, yeast or bacteria. It can be used for multiple applications including protein purification, western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry and other studies.

Components

The main components include sodium fluoride, sodium orthovanadate, sodium pyrophosphate, beta-glycerophosphate, imidazole, sodium molybdate, sodium tartrate dibasic dihydrate, and other inhibitors, mainly inhibiting serine/threonine phosphatases, acid phosphatases, alkaline phosphatases, tyrosine phosphotases, etc.

Storage

Store at -20°C for up to one year.

Usage

Dissolve well at room temperature and add to the lysate or sample solution at a dilution ratio of 1:100 or according to the needs of the experiment.

Notes

1. The phosphatase content can differ across samples. Therefore, the amount of phosphatase inhibitor cocktail can be adjusted according to the experimental situation.

2. This product is a highly concentrated solution. A small amount of precipitation may occur and, therefore, needs to be fully dissolved before use.

3. Dissolve at room temperature, do not heat.

4. For your safety and health, please wear lab coats and disposable gloves.

Cited in Article as

PR20015, Phosphatase Inhibitor Cocktail (100X), Proteintech, IL, USA

Publications

Application	Title
	Life Sci; Gap26 inhibited angiogenesis through the β-catenin-VE-cadherin-VEGFR2-Erk signaling pathway; Authors - Chuyang Xu
	Hum Cell; NR2F1 overexpression alleviates trophoblast cell dysfunction by inhibiting GDF15/MAPK axis in preeclampsia; Authors - Ke Zhang
	BMC Endocr Disord; Acteoside protects podocyte against apoptosis through regulating AKT/GSK-3β signaling pathway in db/db mice; Authors - Xiaoya Li
	Front Pharmacol; Ephedra sinica polysaccharide regulate the anti-inflammatory immunity of intestinal microecology and bacterial metabolites in rheumatoid arthritis; Authors - Yanmiao Ma
	Virus Res; In vitro assessment of the anti-adenoviral activity of artemisinin and its derivatives; Authors - Diyuan Yang
	Int J Biol Macromol; Molecular exploration of the diurnal alteration of glycogen structural fragility and stability in time-restricted-feeding mouse liver; Authors - Zhang-Wen Ma