Phospho-ALK (Tyr1507) Recombinant monoclonal antibody

• 

  WB analysis using 87322-1-RR

  Karpas-299 cell lysates were subjected to SDS PAGE followed by western blot with 87322-1-RR (Phospho-ALK (Tyr1507) antibody) blocked with BSA only, Phospho-ALK (Tyr1507) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Phospho-ALK (Tyr1507) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87322-1-RR

Clone No.252555G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ALK (Tyr1507), NPM-ALK, NPM-ALK (Tyr1507), p-ALK, p-ALK (Tyr1507)

---

Tested Applications

• Positive WB detected in: Karpas-299 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87322-1-RR targets Phospho-ALK (Tyr1507) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: anaplastic lymphoma receptor tyrosine kinase
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC157090
• Gene Symbol: ALK
• Gene ID (NCBI): 238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UM73
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anaplastic lymphoma kinase was first described in 1994 as the NPM-ALK fusion protein that is expressed in the majority of anaplastic large-cell lymphomas. About 2/3rds of anaplastic large-cell lymphomas possess a balanced chromosomal translocation in which the entire nucle-ophosmin (NPM) gene on chromosome 5 is fused to the 3' portion of the ALK gene (including the entire intracellular segment with its protein kinase domain) on chromosome 2. This oncogenic ALK protein kinase is a chimeric protein created by a translocation between chromosomes(2;5)(p23;q35) that generates the NPM-ALK fusion protein. NPM-ALK also known as p80, since the chimeric protein weighs 80 kDa. Studies of NPM-ALK have identified pTyr1096 as the binding site for insulin receptor substrate 1 (IRS1); pTyr1507 is the binding site for Shc and pTyr1604 is the binding site for PLC-Y (native human ALK receptor residue numbers are given; subtract 940 to obtain the NPM-ALK residue). (PMID: 23201355, PMID: 33466277)