Phospho-CD19 (Tyr531) Recombinant monoclonal antibody

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  WB analysis using 87153-1-RR

  Ramos cell lysates were subjected to SDS PAGE followed by western blot with 80351-3-RR (Phospho-CD19 (Tyr531) antibody) blocked with BSA only, Phospho-CD19 (Tyr531) peptide or non-Phospho peptide at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Phospho-CD19 (Tyr531) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87153-1-RR

Clone No.252332G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD19 (Tyr531), p-CD19, p-CD19 (Tyr531), p-CD19(Tyr531), Phospho CD19

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Tested Applications

• Positive WB detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87153-1-RR targets Phospho-CD19 (Tyr531) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: CD19 molecule
• Calculated Molecular Weight: 556 aa, 61 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: NM_001770
• Gene Symbol: CD19
• Gene ID (NCBI): 930
• ENSEMBL Gene ID: ENSG00000177455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15391
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. Phospho-CD19 (Tyr531) is a phosphorylated form of CD19, which is a protein involved in B cell receptor (BCR) signaling and plays a crucial role in B cell development and function. Phosphorylation of CD19 at Tyr531 is known to recruit PI3K activity leading to Akt activation and attendant S473 phosphorylation. This activation of BCR induces multiple oncogenic signals and treatment with ibrutinib prevents preferentially the activation of BTK in both patients with ABC-like and GCB-like molecular features. (PMID: 20038598)