Phospho-CDK9 (Thr186) Recombinant monoclonal antibody

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  WB analysis using 87523-1-RR

  NIH/3T3 cell lysates and C6 cell lysates were subjected to SDS PAGE followed by western blot with 87523-1-RR (Phospho-CDK9 (Thr186) antibody) blocked with BSA only, Phospho-CDK9 (Thr186) peptide or non-Phospho peptide at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Phospho-CDK9 (Thr186) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87523-1-RR

Clone No.252873G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CDK9, C 2k, C-2K, CDC2L4, Cell division cycle 2-like protein kinase 4

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Tested Applications

• Positive WB detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87523-1-RR targets Phospho-CDK9 (Thr186) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cyclin-dependent kinase 9
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001261
• Gene Symbol: CDK9
• Gene ID (NCBI): 1025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50750
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. Phospho-CDK9 (Thr186) is the active-site-switching modification of the catalytic subunit of P-TEFb (positive transcription elongation factor b). Phosphorylation at threonine 186, located in the T-loop of CDK9, is essential for full kinase activity: it swings the T-loop away from the catalytic cleft, allowing ATP and protein substrates (e.g., the C-terminal domain of RNA polymerase II, DSIF and NELF) to enter and be efficiently phosphorylated. Thus, p-Thr186-CDK9 licenses the elongation step of most protein-coding transcripts and is indispensable for productive mRNA synthesis. (PMID: 39800748)