Phospho-Beta Catenin (Ser33) Recombinant antibody

Phospho-Beta Catenin (Ser33) Recombinant Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3K1

Cat no : 80067-1-RR

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286



Tested Applications

Positive WB detected inPC-3 cells, HT-29 cells, Calyculin A treated HT-29 cells, Calyculin A treated PC-3 cells
Positive FC detected inCalyculin A treated PC-3 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

80067-1-RR targets Phospho-Beta Catenin (Ser33) in WB, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC058926
Gene Symbol CTNNB1
Gene ID (NCBI) 1499
RRIDAB_2918861
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines .

Protocols

Product Specific Protocols
WB protocol for Phospho-Beta Catenin (Ser33) antibody 80067-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mediators Inflamm

Liraglutide Attenuates Hepatic Oxidative Stress, Inflammation, and Apoptosis in Streptozotocin-Induced Diabetic Mice by Modulating the Wnt/β-Catenin Signaling Pathway

Authors - Jie Yu
humanWB

Front Pharmacol

Autophagy Blockade by Ai Du Qing Formula Promotes Chemosensitivity of Breast Cancer Stem Cells Via GRP78/β-Catenin/ABCG2 Axis.

Authors - Mianmian Liao
humanWB

Acta Biochim Biophys Sin (Shanghai)

Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide-α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway.

Authors - Qiang Zuo
mouseWB

Exp Ther Med

Insulin and liraglutide attenuate brain pathology in diabetic mice by enhancing the Wnt/β-catenin signaling pathway.

Authors - Yuan Zhao
mouseWB

Environ Toxicol

Microplastics cause hepatotoxicity in diabetic mice by disrupting glucolipid metabolism via PP2A/AMPK/HNF4A and promoting fibrosis via the Wnt/β-catenin pathway

Authors - Xinxin Li