Phospho-FRS2 (Tyr436) Polyclonal antibody

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  WB analysis using 31352-1-AP

  Non-treated NIH/3T3 cells and bFGF (HZ-1285) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 31352-1-AP (Phospho-FRS2 (Tyr436) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

Phospho-FRS2 (Tyr436) Polyclonal Antibody for WB, ELISA

Cat No. 31352-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

p FRS2 , p FRS2 (Tyr436), p FRS2 Tyr436, p-FRS2 , p-FRS2 (Tyr436)

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Tested Applications

• Positive WB detected in: bFGF treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31352-1-AP targets Phospho-FRS2 (Tyr436) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: fibroblast growth factor receptor substrate 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC021562
• Gene Symbol: FRS2
• Gene ID (NCBI): 10818
• RRID: AB_3669956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WU20
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor substrate 2 (FRS2), a lipid-anchored docking protein that is phosphorylated upon activation of FGFR, is critical for recruitment of downstream signaling molecules and links the FGFRs to the Ras/Mek/Erk pathway and the PI3-Kinase/Akt pathway. Studies have found that the phosphotyrosine binding domain of FRS-2 directly binds the Trk receptors at the same phosphotyrosine residue that binds the signaling adapter Shc, suggesting a model in which competitive binding between FRS-2 and Shc regulates differentiation versusproliferation. (PMID: 19053057, PMID: 10092678)