Phospho-Histone H2B (Ser32) Recombinant monoclonal antibody

Phospho-Histone H2B (Ser32) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86873-1-RR
Clone No.242907D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

H2B, H2B Phos Ser32, H2B Phospho S32, H2B Phospho Ser32, H2B Phospho Serine9

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected innocodazole treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86873-1-RR targets Phospho-Histone H2B (Ser32) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name histone cluster 2, H2be
Calculated Molecular Weight 14 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

During mitosis, Aurora-B directly binds and phosphorylates Histone H2B at Ser32. (PMID: 29563611)

Protocols

Product Specific Protocols
WB protocol for Phospho-Histone H2B (Ser32) antibody 86873-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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