Phospho-Histone H3 (Ser10) Recombinant antibody

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  WB analysis of HeLa using 82828-10-RR

  Nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.

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  WB analysis of NIH/3T3 using 82828-10-RR

  Nocodazole treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

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  WB analysis of HSC-T6 using 82828-10-RR

  Calyculin A treated and untreated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 82828-10-RR (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

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  IF Staining of HepG2 using 82828-10-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-Histone H3 (Ser10) antibody (82828-10-RR, Clone: 242921B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 82828-10-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-Histone H3 (Ser10) antibody (82828-10-RR, Clone: 242921B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4, red).

• 

  IF Staining of HepG2 using 82828-10-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-Histone H3 (Ser10) antibody (82828-10-RR, Clone: 242921B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4, red).

Phospho-Histone H3 (Ser10) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 82828-10-RR

Clone No.242921B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

H3 P-S10, H3S10, H3S10Ph, Histone H3 (Ser10), p Histone H3

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Tested Applications

• Positive WB detected in: nocodazole treated HeLa cells, Calyculin A treated HSC-T6 cells, nocodazole NIH/3T3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82828-10-RR targets Phospho-Histone H3 (Ser10) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: NM_003529
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.