Phospho-IRS1 (Ser307) Recombinant antibody, PBS Only

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  WB analysis using 85238-1-RR (same clone as 85238-1-PBS)

  Non-treated NIH/3T3 cells and TNF alpha (HZ-1014) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85238-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85238-1-RR (same clone as 85238-1-PBS)

  Non-treated and TNF alpha (HZ-1014) treated HeLa cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85238-1-PBS in a different storage buffer formulation.

Phospho-IRS1 (Ser307) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85238-1-PBS

Clone No.242124E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

IRS1 (Ser307), P IRS1 (Ser307), Phospho IRS1, Phospho IRS1 (Ser307), Phospho-IRS1

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Product Information

85238-1-PBS targets Phospho-IRS1 (Ser307) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: insulin receptor substrate 1
• Calculated Molecular Weight: 1242 aa, 132 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC053895
• Gene Symbol: IRS1
• Gene ID (NCBI): 3667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35568
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Serine phosphorylation of insulin receptor substrate-1 (IRS-1) inhibits insulin signal transduction in a variety of cell backgrounds, which might contribute to peripheral insulin resistance. However, because of the large number of potential phosphorylation sites, the mechanism of inhibition has been difficult to determine. It has been confirmed that JNK and IKK are involved in the phosphorylation of IRS-1 (Ser307/312). Additionally, activation of Akt, ERK, mTOR, and PKCζ correlates to IRS-1 phosphorylation at Ser307/312, Ser267/270, and Ser612/616 in rodent/human IRS-1. (PMID: 11606564, PMID: 12714600)