Tested Applications
| Positive WB detected in | NIH/3T3 cells, TNF alpha treated HeLa cells, TNF alpha treated NIH/3T3 cells |
This antibody is equivalent to Ser302 in mice.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85238-1-RR targets Phospho-IRS1 (Ser307) in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | insulin receptor substrate 1 |
| Calculated Molecular Weight | 1242 aa, 132 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | BC053895 |
| Gene Symbol | IRS1 |
| Gene ID (NCBI) | 3667 |
| RRID | AB_3744117 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35568 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Serine phosphorylation of insulin receptor substrate-1 (IRS-1) inhibits insulin signal transduction in a variety of cell backgrounds, which might contribute to peripheral insulin resistance. However, because of the large number of potential phosphorylation sites, the mechanism of inhibition has been difficult to determine. It has been confirmed that JNK and IKK are involved in the phosphorylation of IRS-1 (Ser307/312). Additionally, activation of Akt, ERK, mTOR, and PKCζ correlates to IRS-1 phosphorylation at Ser307/312, Ser267/270, and Ser612/616 in rodent/human IRS-1. (PMID: 11606564, PMID: 12714600)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-IRS1 (Ser307) antibody 85238-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-IRS1 (Ser307) Recombinant monoclonal antibody (Cat# 85238-1-RR) has 9 citations. These appear in journals including Advanced Science, Phytomedicine, Journal of Translational Medicine, International Journal of Biological Macromolecules, Current Research in Food Science, Frontiers in Bioengineering and Biotechnology, Pharmaceuticals, Scientific Reports, and Nutrition & Metabolism. The research fields span insulin resistance, obesity, multi-organ fibrosis, diabetic kidney injury, cardiac failure, gut microbiota modulation, and glucose/lipid metabolism.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Activating the Osteoblastic USP26 Pathway Alleviates Multi-Organ Fibrosis by Decreasing Insulin Resistance. | ||
Phytomedicine Perillyl alcohol ameliorates high-fat diet-induced obesity in mice by modulating gut microbiota and activating IRF4-mediated brown fat thermogenesis. | ||
J Transl Med IMPA2 ameliorates high-fat diet-induced obesity by enhancing adaptive thermogenesis in a Ca²⁺-activated mitochondrial biogenesis pathway. | ||
Int J Biol Macromol Characterization of a polysaccharide from Ipomoea batatas and its in vitro hypoglycemic activity. | ||
Curr Res Food Sci Lacticaseibacillus rhamnosus HF01 postbiotics reprogram gut microbial tryptophan metabolism to coordinate enterohepatic barrier-insulin signaling axis. | ||
Front Bioeng Biotechnol Bioinspired cardiac-targeted metal-organic framework nanozyme for modulating inflammatory responses in heart failure with preserved ejection fraction. |
