Phospho-IRS1 (Ser307) Recombinant antibody

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  WB analysis using 85238-1-RR

  Non-treated NIH/3T3 cells and TNF alpha (HZ-1014) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

• 

  WB analysis using 85238-1-RR

  Non-treated and TNF alpha (HZ-1014) treated HeLa cells were subjected to SDS PAGE followed by western blot with 85238-1-RR (Phospho-IRS1 (Ser307) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

Phospho-IRS1 (Ser307) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85238-1-RR

Clone No.242124E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

IRS1 (Ser307), P IRS1 (Ser307), Phospho IRS1, Phospho IRS1 (Ser307), Phospho-IRS1

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, TNF alpha treated HeLa cells, TNF alpha treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85238-1-RR targets Phospho-IRS1 (Ser307) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: insulin receptor substrate 1
• Calculated Molecular Weight: 1242 aa, 132 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC053895
• Gene Symbol: IRS1
• Gene ID (NCBI): 3667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P35568
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine phosphorylation of insulin receptor substrate-1 (IRS-1) inhibits insulin signal transduction in a variety of cell backgrounds, which might contribute to peripheral insulin resistance. However, because of the large number of potential phosphorylation sites, the mechanism of inhibition has been difficult to determine. It has been confirmed that JNK and IKK are involved in the phosphorylation of IRS-1 (Ser307/312). Additionally, activation of Akt, ERK, mTOR, and PKCζ correlates to IRS-1 phosphorylation at Ser307/312, Ser267/270, and Ser612/616 in rodent/human IRS-1. (PMID: 11606564, PMID: 12714600)