Phospho-NF-κB p65 (Ser468) Recombinant antibody

Phospho-NF-κB p65 (Ser468) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


Human, Mouse and More (4)







Cat no : 82335-1-RR


NFKB3, p65, Phospho-NF-κB p65 (Ser468), RELA, Transcription factor p65

Tested Applications

Positive WB detected inCalyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82335-1-RR targets Phospho-NF-κB p65 (Ser468) in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC011603
Gene Symbol RELA
Gene ID (NCBI) 5970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.


Product Specific Protocols
WB protocol for Phospho-NF-κB p65 (Ser468) antibody 82335-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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