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Phospho-NF-κB p65 (Ser468) Recombinant monoclonal antibody, PBS Only (82335-1-PBS)

Phospho-NF-κB p65 (Ser468) Recombinant monoclonal antibody, PBS Only

Name: Phospho-NF-κB p65 (Ser468) Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 82335-1-PBS-100UG

Phospho-NF-κB p65 (Ser468) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA, ChIP-qPCR

Cat No. 82335-1-PBS

Clone No. 6N1

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA, ChIP-qPCR

Synonyms

NFκB (Ser468), NF-κB (Ser468), NF-κB p65 (Ser468), p NF-κB, p NF-κB (Ser468)

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllWBFC (Intra)ChIP-qPCR

WB analysis using 82335-1-RR (same clone as 82335-1-PBS)
Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 82335-1-RR (Phospho-NF-κB p65 (Ser468) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin antibody (66031-1-Ig) and NF-κB p65 antibody (80979-1-RR) subsequently. This data was developed using the same antibody clone with 82335-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 82335-1-RR (Phospho-NF-κB p65 (Ser468) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin antibody (66031-1-Ig) and NF-κB p65 antibody (80979-1-RR) subsequently. This data was developed using the same antibody clone with 82335-1-PBS in a different storage buffer formulation.
FC experiment of NIH/3T3 using 82335-1-RR (same clone as 82335-1-PBS)
1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-NF-κB p65 (Ser468) Recombinant antibody (82335-1-RR, Clone:6N1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82335-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-NF-κB p65 (Ser468) Recombinant antibody (82335-1-RR, Clone:6N1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82335-1-PBS in a different storage buffer formulation.
ChIP experiment of HeLa using 82335-1-RR (same clone as 82335-1-PBS)
Chromatin was prepared from HeLa cells treated with hTNF-α (30 ng/ml, 1 h). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Phospho-NF-κB p65 (Ser468) (82335-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82335-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Chromatin was prepared from HeLa cells treated with hTNF-α (30 ng/ml, 1 h). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Phospho-NF-κB p65 (Ser468) (82335-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82335-1-PBS in a different storage buffer formulation.

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Publications (1)

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Product Information

82335-1-PBS targets Phospho-NF-κB p65 (Ser468) in WB, FC (Intra), Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated Molecular Weight	65 kDa
Observed Molecular Weight	75 kDa
GenBank Accession Number	BC011603
Gene Symbol	NF-κB p65
Gene ID (NCBI)	5970
RRID	AB_3083091
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q04206
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.

Publications

What published studies show

The Phospho-NF-κB p65 (Ser468) Recombinant monoclonal antibody (6N1), catalog number 82335-1-PBS, has a total of 633 citations. A retrieved citation appears in Neoplasia (2025), addressing bone cancer pain through neuronal oxidative stress and microglial inhibition, reflecting research fields spanning oncology, neuroscience, and inflammation. The antibody has been cited across applications including WB, IHC, IF, and ELISA in species such as human, mouse, rat, pig, chicken, bovine, sheep, and goat.

Species	Application	Title
mouse	WB	Neoplasia ZC3H15 suppression ameliorates bone cancer pain through inhibiting neuronal oxidative stress and microglial inflammation Authors - Li-Quan Huang View Article