Tested Applications
| Positive WB detected in | NIH/3T3 cells, PMA treated HL-60 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29123-1-AP targets Phospho-PKC Alpha (Thr638) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | protein kinase C, alpha |
| Calculated Molecular Weight | 77 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | AK055431 |
| Gene Symbol | PKC Alpha |
| Gene ID (NCBI) | 5578 |
| RRID | AB_2918239 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17252 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-PKC Alpha (Thr638) antibody 29123-1-AP | Download protocol |
| WB protocol for Phospho-PKC Alpha (Thr638) antibody 29123-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-PKC Alpha (Thr638) Polyclonal antibody (Cat# 29123-1-AP) has 14 total citations. These appear in journals including Cell Reports Medicine, Molecular Psychiatry, Neurotherapeutics, Biochemical Pharmacology, European Journal of Pharmacology, International Journal of Molecular Sciences, Chemico-Biological Interactions, Poultry Science, Cancer Medicine, Journal of Leukocyte Biology, Cytotechnology, and Journal of Surgical Research. Research fields span oncology (prostate, colorectal, glioma, liver cancer), neurology (Alzheimer's, spinal cord injury), cardiology, hepatology, pharmacology, and immunology, with applications mainly in Western blot and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Cell Rep Med Cholinergic signaling via muscarinic M1 receptor confers resistance to docetaxel in prostate cancer | ||
Mol Psychiatry PIAS1-mediated GSK3β SUMOylation exacerbates tauopathy and cognitive deficits in Alzheimer's disease models. | ||
Neurotherapeutics Regulatory T cells attenuate astrogliosis via IL-10/STAT3/PKC/vimentin signaling and promote neurological recovery after spinal cord injury. | ||
Biochem Pharmacol IGFBP5 suppresses colorectal cancer stemness and carcinogenesis via binding to GPR182 and inhibiting PKCα/AKT/P70S6K axis. | ||
Biochem Pharmacol Sodium butyrate upregulates URAT1-mediated urate reabsorption in HK2 cells by activating FFAR2/p38 MAPK/ELK1/ARRB2 axis and relieving NF-κB-HNF1β competitive binding. | ||
Eur J Pharmacol Salicylic acid inhibits Mas-related G protein-coupled receptor X2-mediated mast cell 2 activation and mitigates cutaneous pseudo-allergic reactions. |
Reviews
What customers say
The single review for this antibody reports a 5-star rating from a Western Blot application on HEK293 cells using a 1:2000 dilution. The reviewer described it as a good antibody that works well at room temperature, indicating reliable performance and convenience in their workflow. Overall, the feedback is positive, highlighting both the product's effectiveness and ease of use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Gaurav (Verified Customer) (12-05-2025) | Good antibody working on room temperature
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