Phospho-PKN1 (Thr774) Polyclonal antibody

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  WB analysis using 29107-1-AP

  Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29107-1-AP (Phospho-PKN1 (Thr774) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours.

Phospho-PKN1 (Thr774) Polyclonal Antibody for WB, ELISA

Cat No. 29107-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

DBK, PAK 1, PAK1, Phospho-PKN1 (Thr774), PKN, PKN ALPHA, PKN1, PRK1, PRKCL1, Protease activated kinase 1, Protein kinase C like 1, Protein kinase C like PKN, protein kinase N1, Protein kinase PKN alpha

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Tested Applications

• Positive WB detected in: PDGF treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29107-1-AP targets Phospho-PKN1 (Thr774) in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein kinase N1
• Calculated Molecular Weight: 942 aa, 104 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC040061
• Gene Symbol: PKN1
• Gene ID (NCBI): 5585
• RRID: AB_2918235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16512
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKN1 is a stress-responsive kinase and a member of the protein kinase novel (PKN) family also known as protein kinase C-related kinases (PRKs). PKN1 acting downstream of small GTP-binding proteins of the Rho/Rac family. The interaction of Rho with PKN1 induces a conformational change in PKN1 leading to activation loop phosphorylation by PDK1 on Thr774 in the case of human PKN1 (Thr778 in mouse/rat) which is necessary for the catalytic activation of PKN1/2 and critical for the stability of the protein. (PMID: 29045568, PMID: 21037231)