Phospho-PRKD1 (Ser916) Polyclonal antibody

Phospho-PRKD1 (Ser916) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28928-1-AP

Synonyms

Phospho-PRKD1 (Ser916), PKC MU, PKCM, PKD, PKD1, PRKCM, PRKD1, Protein kinase C mu type, Protein kinase D, protein kinase D1

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Validation Data Gallery

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  WB analysis using 28928-1-AP

  Non-treated NIH/3T3 and Insulin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28928-1-AP (Phospho-PRKD1 (Ser916) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

• Positive WB detected in: Insulin treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

28928-1-AP targets Phospho-PRKD1 (Ser916) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase D1
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_001330069
• Gene Symbol: PRKD1
• Gene ID (NCBI): 5587
• RRID: AB_2918215
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.1% sodium azide, 50% glycerol pH 7.3 and 0.05%BSA.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase D1 (PRKD1), also named as PKD1 and PKCμ, is comprised of two cysteine-rich domains and a pleckstrin homology (PH) domain. PKD1 is involved in cellular processes including protein secretion, proliferation, cytoskeletal reorganization, Golgi function, immune function and apoptosis. It is widely expressed in thyroid, brain, heart, lung and other tissues. PKCs have been shown to regulate PKD1 activation. It has been reported that ser 916 is a PKD1 autophosphorylation site. PKD1 can be activated by growth factors, oxidative stress, thrombin, bioactive lipids, cross-linking of B- and T-cell receptors and some G-protein coupled receptors (GPCR). PKD1 is located mainly in the cytoplasm in unstimulated cells, while PKD1 migrates to the membrane in activated cells. (PMID: 17306383, 24806360, 30101477, 21696630)

Publications

Species	Application	Title
rat	WB	Food Chem Toxicol; G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes.; Authors - Yao Liu
rat	WB	Biochem Biophys Res Commun; Protein kinase D1 promotes the survival of random-pattern skin flaps in rats; Authors - Jianpeng Chen