Phospho-TAU (Thr205) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86669-1-RR (same clone as 86669-1-PBS)

  Phosphatase inhibitor treated and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 86669-1-RR (Phospho-TAU (Thr205) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 86669-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86669-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86669-1-RR (Phospho-TAU (Thr205) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Phospho-TAU (Thr205) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86669-1-PBS

Clone No.251173E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

MAPT, TAU, AT180, DDPAC, MAPTL

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Product Information

86669-1-PBS targets Phospho-TAU (Thr205) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: microtubule-associated protein tau
• Calculated Molecular Weight: 37-46, 79-81 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: BC000558
• Gene Symbol: TAU
• Gene ID (NCBI): 4137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10636
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.