Phospho-VASP (Ser157) Recombinant monoclonal antibody

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  WB analysis of NIH/3T3 using 87168-2-RR

  Non-treated NIH/3T3 cells and Forskolin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87168-2-RR (Phospho-VASP(Ser157) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-VASP (Ser157) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87168-2-RR

Clone No.252276B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Phospho VASP (Ser157), Phospho-VASP, Phospho-VASP-Ser157, p-VASP (S157), p-VASP (Ser157)

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Tested Applications

• Positive WB detected in: Forskolin treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87168-2-RR targets Phospho-VASP (Ser157) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: vasodilator-stimulated phosphoprotein
• Calculated Molecular Weight: 380 aa, 40 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC038224
• Gene Symbol: VASP
• Gene ID (NCBI): 7408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50552
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VASP belongs to the Ena/VASP family. Phospho-VASP (Ser157) is an important post-translational modification of the vasodilator-stimulated phosphoprotein (VASP). Phosphorylation at Serine 157 (Ser157) is catalyzed by protein kinase C (PKC) and Rho kinase in response to thrombin or phorbol ester stimulation in human platelets. This modification negatively regulates platelet adhesion and secretion, and serves as a key signaling event in modulating cytoskeletal dynamics and cell motility. (PMID: 16197368)