Phospho-ZAP70 (Tyr319)/SYK (Tyr352) Recombinant monoclonal antibody

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  WB analysis of Jurkat using 81767-2-RR

  Non-treated Jurkat cells and H2O2 treated Jurkat cells were subjected to SDS PAGE followed by western blot with 81767-2-RR (Phospho-ZAP70 (Tyr319)/Syk (Tyr352) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-ZAP70 (Tyr319)/SYK (Tyr352) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 81767-2-RR

Clone No.250683A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho Syk (Tyr352), Phospho ZAP70 (Tyr319)/Syk (Tyr352), Phospho Zap-70 (Tyr319)/Syk (Tyr352), Phospho-Syk (Tyr352), Phospho-Syk-Tyr352

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Tested Applications

• Positive WB detected in: H2O2 treated Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81767-2-RR targets Phospho-ZAP70 (Tyr319)/SYK (Tyr352) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: zeta-chain (TCR) associated protein kinase 70kDa
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa, 72kDa
• GenBank Accession Number: BC039039
• Gene Symbol: ZAP70
• Gene ID (NCBI): 7535
• ENSEMBL Gene ID: ENSG00000115085
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43403
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. Three tyrosine residues (Tyr292, Tyr315 and Tyr319) in the interdomain B region of ZAP‐70 have attracted particular attention as sites that might regulate ZAP‐70 activity and/or binding to heterologous proteins. The third candidate phosphorylation site in the interdomain B region of ZAP‐70 is Tyr319. Although the functional importance of this site in ZAP‐70 is unknown, a previous report presented indirect evidence that the orthologous Tyr352 residue in Syk is involved in the association of PLC‐γ1 with this PTK. (PMID: 10202147)