Phospho-eEF2 (Thr57) Recombinant antibody

Phospho-eEF2 (Thr57) Uni-rAbTM Recombinant Antibody for WB, ELISA

Cat No. 80351-2-RR
Clone No.250222A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

p eEF2K, p eEF2K (Thr57), p-eEF2K, p-eEF2K (Thr57), p-eEF2K(Thr57)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80351-2-RR targets Phospho-eEF2 (Thr57) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name eukaryotic elongation factor-2 kinase
Calculated Molecular Weight 725 aa, 100 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC032665
Gene Symbol EEF2K
Gene ID (NCBI) 29904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

eEF2 (Eukaryotic Elongation Factor 2) is a key component in the process of protein synthesis in eukaryotic cells. It is a GTP-binding protein that plays a crucial role in the elongation phase of translation. Specifically, eEF2 facilitates the movement of the ribosome along the mRNA, allowing the addition of amino acids to the growing polypeptide chain. This elongation factor is essential for efficient and accurate protein synthesis. Interestingly, eEF2 phosphorylation can either be increased or decreased depending on the stimulus used in learning or synaptic plasticity paradigms. Examining the specific contributions of increases and decreases in eEF2 phosphorylation to learning and memory is difficult. (PMID: 25762683)

Protocols

Product Specific Protocols
WB protocol for Phospho-eEF2 (Thr57) antibody 80351-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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