CALCA/Calcitonin Recombinant monoclonal antibody, PBS Only

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  IF Staining of TT using 83774-1-RR (same clone as 83774-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed TT cells using Procalcitonin/CALCA antibody (83774-1-RR, Clone: 240753G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).(negative control: HeLa cells) This data was developed using the same antibody clone with 83774-1-PBS in a different storage buffer formulation.

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  FC experiment of TT using 83774-1-RR (same clone as 83774-1-PBS)

  1x10^6 TT cells were intracellularly stained with 0.25 ug Procalcitonin/CALCA Recombinant Antibody (83774-1-RR, Clone:240753G2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83774-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83774-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83774-1-PBS against Human CALCA/Calcitonin was performed. The affinity constant is 47.7 nM.

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  Affinity and Kinetic Characterization of 83774-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83774-1-RR against Human CALCA/CGRP were performed. The affinity constant is 0.189 nM.

CALCA/Calcitonin Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83774-1-PBS

Clone No.240753G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Procalcitonin/CALCA, CALCA, Calcitonin, 240753G2, CALC1

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Product Information

83774-1-PBS targets CALCA/Calcitonin in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1060

  Product name: Recombinant Human Procalcitonin/CALCA protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 26-141 aa of NM_001741.3

  Sequence: APFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAPGKKRDMSSDLERDHRPHVSMPQNAN

• Full Name: Calcitonin
• Calculated Molecular Weight: 15kDa
• GenBank Accession Number: NM_001741.3
• Gene Symbol: CALCA
• Gene ID (NCBI): 796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01258
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.