Progranulin/PGRN Recombinant antibody

• 

  WB analysis using 86118-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86118-2-RR (GRN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Progranulin/PGRN Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86118-2-RR

Clone No.250681G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

granulin, Epithelin precursor, Glycoprotein 88, Glycoprotein of 88 Kda, Granulin precursor

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293T cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86118-2-RR targets Progranulin/PGRN in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Progranulin/PGRN fusion protein Eg2954
• Full Name: granulin
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: NM_002087.3
• Gene Symbol: Granulin
• Gene ID (NCBI): 2896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28799-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.