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RAB1B Polyclonal antibody

RAB1B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17824-1-AP

Synonyms

RAB1B, Ras related protein Rab 1B



Tested Applications

Positive WB detected inA375 cells, HeLa cells, HAP1, A431 cells, NIH/3T3 cells
Positive IHC detected inhuman heart tissue, mouse heart tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17824-1-AP targets RAB1B in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB1B fusion protein Ag12197
Full Name RAB1B, member RAS oncogene family
Calculated molecular weight 201 aa, 22 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC071169
Gene symbol RAB1B
Gene ID (NCBI) 81876
RRIDAB_2237881
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAB1B antibody 17824-1-APDownload protocol
IHC protocol for RAB1B antibody 17824-1-APDownload protocol
IF protocol for RAB1B antibody 17824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.

Authors - Janice D Thomas
humanWB

Nat Cell Biol

A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.

Authors - Reiling Jan H JH
humanIF

Dev Cell

SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.

Authors - Rui Yan
humanIHC

Aging (Albany NY)

Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer.

Authors - Xian-Zi Yang
ratWB

Front Pharmacol

Fuyuan Xingnao Decoction Promotes Angiogenesis Through the Rab1/AT1R Pathway in Diabetes Mellitus Complicated With Cerebral Infarction.

Authors - Dong Deng
humanWB

Oncol Rep

Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer.

Authors - Hao Bo