RAB1B Polyclonal antibody

RAB1B Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 17824-1-AP

Synonyms

RAB1B, Ras related protein Rab 1B

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Validation Data Gallery

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  WB analysis of HeLa using 17824-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 17824-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17824-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17824-1-AP (RAB1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 17824-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17824-1-AP (RAB1B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 17824-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 17824-1-AP (RAB1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, A375 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

17824-1-AP targets RAB1B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB1B fusion protein Ag12197
• Full Name: RAB1B, member RAS oncogene family
• Calculated molecular weight: 201 aa, 22 kDa
• Observed molecular weight: 22-25 kDa
• GenBank accession number: BC071169
• Gene symbol: RAB1B
• Gene ID (NCBI): 81876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Cell; Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.; Authors - Janice D Thomas
human	WB	Nat Cell Biol; A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.; Authors - Reiling Jan H JH
human	IF	Dev Cell; SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.; Authors - Rui Yan
human	IHC	Aging (Albany NY); Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer.; Authors - Xian-Zi Yang
rat	WB	Front Pharmacol; Fuyuan Xingnao Decoction Promotes Angiogenesis Through the Rab1/AT1R Pathway in Diabetes Mellitus Complicated With Cerebral Infarction.; Authors - Dong Deng
human	WB	Oncol Rep; Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer.; Authors - Hao Bo