RAB1B Polyclonal antibody

RAB1B Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17824-1-AP

Synonyms

RAB1B, Ras related protein Rab 1B



Tested Applications

Positive WB detected inHeLa cells, A431 cells, A375 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17824-1-AP targets RAB1B in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB1B fusion protein Ag12197
Full Name RAB1B, member RAS oncogene family
Calculated molecular weight 201 aa, 22 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC071169
Gene symbol RAB1B
Gene ID (NCBI) 81876
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB1B antibody 17824-1-APDownload protocol
IHC protocol for RAB1B antibody 17824-1-APDownload protocol
IF protocol for RAB1B antibody 17824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cancer

High Expression of lncRNA AFAP1-AS1 Promotes the Progression of Colon Cancer and Predicts Poor Prognosis.

Authors - Hao Bo
pigWB

Vet Microbiol

Rab1b-GBF1-ARFs mediated intracellular trafficking is required for classical swine fever virus replication in swine umbilical vein endothelial cells.

Authors - Liang Zhang
ratWB

Front Pharmacol

Fuyuan Xingnao Decoction Promotes Angiogenesis Through the Rab1/AT1R Pathway in Diabetes Mellitus Complicated With Cerebral Infarction.

Authors - Dong Deng
humanIHC

Aging (Albany NY)

Overexpression of Rab1B and MMP9 predicts poor survival and good response to chemotherapy in patients with colorectal cancer.

Authors - Xian-Zi Yang
humanWB

Nat Cell Biol

A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.

Authors - Reiling Jan H JH
humanWB

Cancer Cell

Rab1A Is an mTORC1 Activator and a Colorectal Oncogene.

Authors - Janice D Thomas